Quantitative analysis of propionibacterial DNA in bronchoalveolar lavage cells from patients with sarcoidosis

Hirohisa Ichikawa, M. Kataoka, J. Hiramatsu, M. Ohmori, Y. Tanimoto, A. Kanehiro, Y. Nakata, M. Tanimoto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background and aim of the work: The causes of sarcoidosis are still unknown. Propionibacterial subspieces are thought to be one of the most likely sources of antigens. Here we attempted to measure the amount of propionibacterial DNA in bronchoalveolar lavage (BAL) cell samples from patients with sarcoidosis and other pulmonary diseases. Methods: We examined BAL cells from 42 patients with sarcoidosis and 30 controls. Using quantitative polymerase chain reaction (PCR) for 16S rRNA of Propionibacterium acnes (P. acnes) and Propionibacterium granulosum (P. granulosum), we measured the amount of propionibacterial DNA in 500 ng of total DNA extracted from BAL cells from patients with sarcoidosis or other lung diseases. The correlation between clinical findings and the results of quantitative PCR were analyzed. Results: The mean level of P. acnes DNA from patients with sarcoidosis was 59.9 genomes per 500 ng of total DNA, which was significantly higher than that in controls (20.7 genomes, p

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalSarcoidosis Vasculitis and Diffuse Lung Diseases
Volume25
Issue number1
Publication statusPublished - Sep 2008

Fingerprint

Bronchoalveolar Lavage
Sarcoidosis
DNA
Propionibacterium acnes
Lung Diseases
Genome
Propionibacterium
Polymerase Chain Reaction
Antigens

Keywords

  • Bronchoalveolar lavage cells
  • P. acnes
  • P. granulosum
  • Quantitative PCR
  • Sarcoidosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Immunology and Allergy
  • Internal Medicine

Cite this

Ichikawa, H., Kataoka, M., Hiramatsu, J., Ohmori, M., Tanimoto, Y., Kanehiro, A., ... Tanimoto, M. (2008). Quantitative analysis of propionibacterial DNA in bronchoalveolar lavage cells from patients with sarcoidosis. Sarcoidosis Vasculitis and Diffuse Lung Diseases, 25(1), 15-20.

Quantitative analysis of propionibacterial DNA in bronchoalveolar lavage cells from patients with sarcoidosis. / Ichikawa, Hirohisa; Kataoka, M.; Hiramatsu, J.; Ohmori, M.; Tanimoto, Y.; Kanehiro, A.; Nakata, Y.; Tanimoto, M.

In: Sarcoidosis Vasculitis and Diffuse Lung Diseases, Vol. 25, No. 1, 09.2008, p. 15-20.

Research output: Contribution to journalArticle

Ichikawa, H, Kataoka, M, Hiramatsu, J, Ohmori, M, Tanimoto, Y, Kanehiro, A, Nakata, Y & Tanimoto, M 2008, 'Quantitative analysis of propionibacterial DNA in bronchoalveolar lavage cells from patients with sarcoidosis', Sarcoidosis Vasculitis and Diffuse Lung Diseases, vol. 25, no. 1, pp. 15-20.
Ichikawa, Hirohisa ; Kataoka, M. ; Hiramatsu, J. ; Ohmori, M. ; Tanimoto, Y. ; Kanehiro, A. ; Nakata, Y. ; Tanimoto, M. / Quantitative analysis of propionibacterial DNA in bronchoalveolar lavage cells from patients with sarcoidosis. In: Sarcoidosis Vasculitis and Diffuse Lung Diseases. 2008 ; Vol. 25, No. 1. pp. 15-20.
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