Quantitative analysis of p40/p46 and p69/p71 forms of 2′,5′-oligoadenylate synthetase mRNA by competitive PCR and its clinical application

Akira Takahashi, Yoshiaki Iwasaki, Jiro Miyaike, Hideaki Taniguchi, Hiroyuki Shimomura, Tadashi Hanafusa, Yasuhiro Yumoto, Akio Moriya, Norio Koide, Takao Tsuji

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Abstract

Background: 2′,5′-Oligoadenylate synthetases (2-5AS) are type I interferon (IFN)-induced proteins with antiviral capacity. Three major forms of 2-5AS with distinct enzymatic activities have been described in IFN-treated human cells. We measured distinct forms of 2-5AS mRNA to analyze the relationship with its enzymatic activity and response to IFN therapy in chronic hepatitis C. Methods: We established a method to quantify p40/p46 and p69/p71 forms of 2-5AS mRNA by use of reverse transcription followed by competitive PCR. The 2-5AS mRNA concentrations were measured in peripheral blood mononuclear cells from 40 patients with chronic hepatitis C and 28 control individuals. Results: Reconstitution experiments and comparison with Northern blot analyses revealed that our method accurately and linearly quantified 2-5AS mRNA. 2-5AS mRNA concentrations and 2-5AS enzymatic activity were correlated (P

Original languageEnglish
Pages (from-to)1551-1559
Number of pages9
JournalClinical Chemistry
Volume48
Issue number9
Publication statusPublished - Sep 2002

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ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Takahashi, A., Iwasaki, Y., Miyaike, J., Taniguchi, H., Shimomura, H., Hanafusa, T., Yumoto, Y., Moriya, A., Koide, N., & Tsuji, T. (2002). Quantitative analysis of p40/p46 and p69/p71 forms of 2′,5′-oligoadenylate synthetase mRNA by competitive PCR and its clinical application. Clinical Chemistry, 48(9), 1551-1559.