Quantitative analysis of p40/p46 and p69/p71 forms of 2′,5′-oligoadenylate synthetase mRNA by competitive PCR and its clinical application

Akira Takahashi, Yoshiaki Iwasaki, Jiro Miyaike, Hideaki Taniguchi, Hiroyuki Shimomura, Tadashi Hanafusa, Yasuhiro Yumoto, Akio Moriya, Norio Koide, Takao Tsuji

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: 2′,5′-Oligoadenylate synthetases (2-5AS) are type I interferon (IFN)-induced proteins with antiviral capacity. Three major forms of 2-5AS with distinct enzymatic activities have been described in IFN-treated human cells. We measured distinct forms of 2-5AS mRNA to analyze the relationship with its enzymatic activity and response to IFN therapy in chronic hepatitis C. Methods: We established a method to quantify p40/p46 and p69/p71 forms of 2-5AS mRNA by use of reverse transcription followed by competitive PCR. The 2-5AS mRNA concentrations were measured in peripheral blood mononuclear cells from 40 patients with chronic hepatitis C and 28 control individuals. Results: Reconstitution experiments and comparison with Northern blot analyses revealed that our method accurately and linearly quantified 2-5AS mRNA. 2-5AS mRNA concentrations and 2-5AS enzymatic activity were correlated (P

Original languageEnglish
Pages (from-to)1551-1559
Number of pages9
JournalClinical Chemistry
Volume48
Issue number9
Publication statusPublished - Sep 2002

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Ligases
Polymerase Chain Reaction
Messenger RNA
Chemical analysis
Chronic Hepatitis C
Interferons
Interferon Type I
adjuvant P40
2',5'-oligoadenylate
Transcription
Northern Blotting
Reverse Transcription
Antiviral Agents
Blood Cells
Blood
Cells

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Quantitative analysis of p40/p46 and p69/p71 forms of 2′,5′-oligoadenylate synthetase mRNA by competitive PCR and its clinical application. / Takahashi, Akira; Iwasaki, Yoshiaki; Miyaike, Jiro; Taniguchi, Hideaki; Shimomura, Hiroyuki; Hanafusa, Tadashi; Yumoto, Yasuhiro; Moriya, Akio; Koide, Norio; Tsuji, Takao.

In: Clinical Chemistry, Vol. 48, No. 9, 09.2002, p. 1551-1559.

Research output: Contribution to journalArticle

Takahashi, A, Iwasaki, Y, Miyaike, J, Taniguchi, H, Shimomura, H, Hanafusa, T, Yumoto, Y, Moriya, A, Koide, N & Tsuji, T 2002, 'Quantitative analysis of p40/p46 and p69/p71 forms of 2′,5′-oligoadenylate synthetase mRNA by competitive PCR and its clinical application', Clinical Chemistry, vol. 48, no. 9, pp. 1551-1559.
Takahashi, Akira ; Iwasaki, Yoshiaki ; Miyaike, Jiro ; Taniguchi, Hideaki ; Shimomura, Hiroyuki ; Hanafusa, Tadashi ; Yumoto, Yasuhiro ; Moriya, Akio ; Koide, Norio ; Tsuji, Takao. / Quantitative analysis of p40/p46 and p69/p71 forms of 2′,5′-oligoadenylate synthetase mRNA by competitive PCR and its clinical application. In: Clinical Chemistry. 2002 ; Vol. 48, No. 9. pp. 1551-1559.
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AU - Takahashi, Akira

AU - Iwasaki, Yoshiaki

AU - Miyaike, Jiro

AU - Taniguchi, Hideaki

AU - Shimomura, Hiroyuki

AU - Hanafusa, Tadashi

AU - Yumoto, Yasuhiro

AU - Moriya, Akio

AU - Koide, Norio

AU - Tsuji, Takao

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