Quantitative analysis of cyclin D1 messenger RNA expression in head and neck squamous cell carcinomas

Nobuya Monden, Kazunori Nishizaki, Kunihiro Fukushima, Yu Masuda, Kazunori Tsukuda, Kenji Shimizu

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Cyclin D1 is thought to play a critical role in the G1/S phase transition of the cell cycle. Amplification of this gene has been reported in several types of human neoplasms including breast, lung, esophageal, and head and neck tumors. In this study, we have analyzed the relative level of expression of cyclin D1 messenger RNA (mRNA) in fresh specimens of head and neck squamous cell carcinoma (HNSCC), and investigated the concordance of the overexpression of cyclin D1 mRNA with gene amplification. Levels of cyclin D1 mRNA were analyzed by a modified method of competitive reverse transcription-polymerase chain reaction and levels of cyclin D1 gene amplification were evaluated by Southern blot hybridization in a series of 23 matched normal mucosas and HNSCC. Overexpression of cyclin D1 mRNA was observed in 10 of 23 cases (43.5%) of HNSCC, ranging from 2 to 50-fold higher than the normal control. Twelve of 23 cases could be evaluated by Southern blot hybridization, and gene amplification was found in only 2 of 12 cases (16.7%). These findings suggest that cyclin D1 plays an important role in tumorigenesis of HNSCC, and gene amplification is not one of the major mechanisms for overexpression of cyclin D1.

Original languageEnglish
Pages (from-to)660-668
Number of pages9
JournalJapanese Journal of Cancer Research
Issue number7
Publication statusPublished - Jul 1997
Externally publishedYes


  • Competitive RT-PCR
  • Cyclin D1
  • Head and neck squamous cell carcinoma
  • Quantitative analysis
  • Southern blot hybridization

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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