Abstract
Cyclin D1 is thought to play a critical role in the G1/S phase transition of the cell cycle. Amplification of this gene has been reported in several types of human neoplasms including breast, lung, esophageal, and head and neck tumors. In this study, we have analyzed the relative level of expression of cyclin D1 messenger RNA (mRNA) in fresh specimens of head and neck squamous cell carcinoma (HNSCC), and investigated the concordance of the overexpression of cyclin D1 mRNA with gene amplification. Levels of cyclin D1 mRNA were analyzed by a modified method of competitive reverse transcription-polymerase chain reaction and levels of cyclin D1 gene amplification were evaluated by Southern blot hybridization in a series of 23 matched normal mucosas and HNSCC. Overexpression of cyclin D1 mRNA was observed in 10 of 23 cases (43.5%) of HNSCC, ranging from 2 to 50-fold higher than the normal control. Twelve of 23 cases could be evaluated by Southern blot hybridization, and gene amplification was found in only 2 of 12 cases (16.7%). These findings suggest that cyclin D1 plays an important role in tumorigenesis of HNSCC, and gene amplification is not one of the major mechanisms for overexpression of cyclin D1.
| Original language | English |
|---|---|
| Pages (from-to) | 660-668 |
| Number of pages | 9 |
| Journal | Japanese Journal of Cancer Research |
| Volume | 88 |
| Issue number | 7 |
| Publication status | Published - Jul 1997 |
Fingerprint
Keywords
- Competitive RT-PCR
- Cyclin D1
- Head and neck squamous cell carcinoma
- Quantitative analysis
- Southern blot hybridization
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Quantitative analysis of cyclin D1 messenger RNA expression in head and neck squamous cell carcinomas. / Monden, Nobuya; Nishizaki, Kazunori; Fukushima, Kunihiro; Masuda, Yu; Tsukuda, Kazunori; Shimizu, Kenji.
In: Japanese Journal of Cancer Research, Vol. 88, No. 7, 07.1997, p. 660-668.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative analysis of cyclin D1 messenger RNA expression in head and neck squamous cell carcinomas
AU - Monden, Nobuya
AU - Nishizaki, Kazunori
AU - Fukushima, Kunihiro
AU - Masuda, Yu
AU - Tsukuda, Kazunori
AU - Shimizu, Kenji
PY - 1997/7
Y1 - 1997/7
N2 - Cyclin D1 is thought to play a critical role in the G1/S phase transition of the cell cycle. Amplification of this gene has been reported in several types of human neoplasms including breast, lung, esophageal, and head and neck tumors. In this study, we have analyzed the relative level of expression of cyclin D1 messenger RNA (mRNA) in fresh specimens of head and neck squamous cell carcinoma (HNSCC), and investigated the concordance of the overexpression of cyclin D1 mRNA with gene amplification. Levels of cyclin D1 mRNA were analyzed by a modified method of competitive reverse transcription-polymerase chain reaction and levels of cyclin D1 gene amplification were evaluated by Southern blot hybridization in a series of 23 matched normal mucosas and HNSCC. Overexpression of cyclin D1 mRNA was observed in 10 of 23 cases (43.5%) of HNSCC, ranging from 2 to 50-fold higher than the normal control. Twelve of 23 cases could be evaluated by Southern blot hybridization, and gene amplification was found in only 2 of 12 cases (16.7%). These findings suggest that cyclin D1 plays an important role in tumorigenesis of HNSCC, and gene amplification is not one of the major mechanisms for overexpression of cyclin D1.
AB - Cyclin D1 is thought to play a critical role in the G1/S phase transition of the cell cycle. Amplification of this gene has been reported in several types of human neoplasms including breast, lung, esophageal, and head and neck tumors. In this study, we have analyzed the relative level of expression of cyclin D1 messenger RNA (mRNA) in fresh specimens of head and neck squamous cell carcinoma (HNSCC), and investigated the concordance of the overexpression of cyclin D1 mRNA with gene amplification. Levels of cyclin D1 mRNA were analyzed by a modified method of competitive reverse transcription-polymerase chain reaction and levels of cyclin D1 gene amplification were evaluated by Southern blot hybridization in a series of 23 matched normal mucosas and HNSCC. Overexpression of cyclin D1 mRNA was observed in 10 of 23 cases (43.5%) of HNSCC, ranging from 2 to 50-fold higher than the normal control. Twelve of 23 cases could be evaluated by Southern blot hybridization, and gene amplification was found in only 2 of 12 cases (16.7%). These findings suggest that cyclin D1 plays an important role in tumorigenesis of HNSCC, and gene amplification is not one of the major mechanisms for overexpression of cyclin D1.
KW - Competitive RT-PCR
KW - Cyclin D1
KW - Head and neck squamous cell carcinoma
KW - Quantitative analysis
KW - Southern blot hybridization
UR - http://www.scopus.com/inward/record.url?scp=0030868164&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030868164&partnerID=8YFLogxK
M3 - Article
C2 - 9310139
AN - SCOPUS:0030868164
VL - 88
SP - 660
EP - 668
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 7
ER -