Quantitative analysis of allantoin in fresh tubers of Dioscorea opposita 'Tsukuneimo'

Akio Ninomiya, Yoshiyuki Murata, Mikiro Tada, Yasuaki Shimoishi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Allantoin in fresh tuber of Dioscorea opposita 'Tsukuneimo' was quantitatively analyzed because the compound is thought to prevent inflammation and ulcers in humans as well as to play an important role for storage and translocation of nitrogen in higher plants. The content of ureide (allantoin and allantoic acid) in 'Tsukuneimo' was determined by the modified method of spectrophotometry with phenylhydrazine, whereas the content of allantoin was determined by HPLC. Analyses revealed that 'Tsukuneimo' tubers contained 2.6 mg·gFW-1 of allantoin, but leaves or stems had undetectable levels. D. opposita, 'Yamatoimo' and 'Nagaimo', and D. japonica 'Jinenjo' contained 2.3, 0.47 and 1.2 mg·gFW-1 of allantoin in the tuber, respectively, but negligible amount of allantoic acid in the tuber. Furthermore, Ipomoea batatas and Solanum tuberosum contained less than 0.1 mg·gFW-1 of allantoin and a negligible amount of allantoic acid. Colocasia antiquorum, 'Ishikawawase' and 'Kyouimo' had no detectable amounts of allantoin or allantoic acid.

Original languageEnglish
Pages (from-to)321-323
Number of pages3
JournalJournal of the Japanese Society for Horticultural Science
Volume72
Issue number4
Publication statusPublished - Jul 2003

Fingerprint

Dioscorea oppositifolia
allantoin
allantoic acid
quantitative analysis
tubers
ureides
Colocasia esculenta
Ipomoea batatas
Solanum tuberosum
spectroscopy
inflammation
stems

Keywords

  • Allantoin
  • Dioscorea opposita
  • Tsukuneimo
  • Tuber
  • Ureide

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

Cite this

Quantitative analysis of allantoin in fresh tubers of Dioscorea opposita 'Tsukuneimo'. / Ninomiya, Akio; Murata, Yoshiyuki; Tada, Mikiro; Shimoishi, Yasuaki.

In: Journal of the Japanese Society for Horticultural Science, Vol. 72, No. 4, 07.2003, p. 321-323.

Research output: Contribution to journalArticle

@article{495a828b523b42f7b722dbdbe80d9f16,
title = "Quantitative analysis of allantoin in fresh tubers of Dioscorea opposita 'Tsukuneimo'",
abstract = "Allantoin in fresh tuber of Dioscorea opposita 'Tsukuneimo' was quantitatively analyzed because the compound is thought to prevent inflammation and ulcers in humans as well as to play an important role for storage and translocation of nitrogen in higher plants. The content of ureide (allantoin and allantoic acid) in 'Tsukuneimo' was determined by the modified method of spectrophotometry with phenylhydrazine, whereas the content of allantoin was determined by HPLC. Analyses revealed that 'Tsukuneimo' tubers contained 2.6 mg·gFW-1 of allantoin, but leaves or stems had undetectable levels. D. opposita, 'Yamatoimo' and 'Nagaimo', and D. japonica 'Jinenjo' contained 2.3, 0.47 and 1.2 mg·gFW-1 of allantoin in the tuber, respectively, but negligible amount of allantoic acid in the tuber. Furthermore, Ipomoea batatas and Solanum tuberosum contained less than 0.1 mg·gFW-1 of allantoin and a negligible amount of allantoic acid. Colocasia antiquorum, 'Ishikawawase' and 'Kyouimo' had no detectable amounts of allantoin or allantoic acid.",
keywords = "Allantoin, Dioscorea opposita, Tsukuneimo, Tuber, Ureide",
author = "Akio Ninomiya and Yoshiyuki Murata and Mikiro Tada and Yasuaki Shimoishi",
year = "2003",
month = "7",
language = "English",
volume = "72",
pages = "321--323",
journal = "Horticulture Journal",
issn = "2189-0102",
publisher = "Japanese Society for Horticultural Science",
number = "4",

}

TY - JOUR

T1 - Quantitative analysis of allantoin in fresh tubers of Dioscorea opposita 'Tsukuneimo'

AU - Ninomiya, Akio

AU - Murata, Yoshiyuki

AU - Tada, Mikiro

AU - Shimoishi, Yasuaki

PY - 2003/7

Y1 - 2003/7

N2 - Allantoin in fresh tuber of Dioscorea opposita 'Tsukuneimo' was quantitatively analyzed because the compound is thought to prevent inflammation and ulcers in humans as well as to play an important role for storage and translocation of nitrogen in higher plants. The content of ureide (allantoin and allantoic acid) in 'Tsukuneimo' was determined by the modified method of spectrophotometry with phenylhydrazine, whereas the content of allantoin was determined by HPLC. Analyses revealed that 'Tsukuneimo' tubers contained 2.6 mg·gFW-1 of allantoin, but leaves or stems had undetectable levels. D. opposita, 'Yamatoimo' and 'Nagaimo', and D. japonica 'Jinenjo' contained 2.3, 0.47 and 1.2 mg·gFW-1 of allantoin in the tuber, respectively, but negligible amount of allantoic acid in the tuber. Furthermore, Ipomoea batatas and Solanum tuberosum contained less than 0.1 mg·gFW-1 of allantoin and a negligible amount of allantoic acid. Colocasia antiquorum, 'Ishikawawase' and 'Kyouimo' had no detectable amounts of allantoin or allantoic acid.

AB - Allantoin in fresh tuber of Dioscorea opposita 'Tsukuneimo' was quantitatively analyzed because the compound is thought to prevent inflammation and ulcers in humans as well as to play an important role for storage and translocation of nitrogen in higher plants. The content of ureide (allantoin and allantoic acid) in 'Tsukuneimo' was determined by the modified method of spectrophotometry with phenylhydrazine, whereas the content of allantoin was determined by HPLC. Analyses revealed that 'Tsukuneimo' tubers contained 2.6 mg·gFW-1 of allantoin, but leaves or stems had undetectable levels. D. opposita, 'Yamatoimo' and 'Nagaimo', and D. japonica 'Jinenjo' contained 2.3, 0.47 and 1.2 mg·gFW-1 of allantoin in the tuber, respectively, but negligible amount of allantoic acid in the tuber. Furthermore, Ipomoea batatas and Solanum tuberosum contained less than 0.1 mg·gFW-1 of allantoin and a negligible amount of allantoic acid. Colocasia antiquorum, 'Ishikawawase' and 'Kyouimo' had no detectable amounts of allantoin or allantoic acid.

KW - Allantoin

KW - Dioscorea opposita

KW - Tsukuneimo

KW - Tuber

KW - Ureide

UR - http://www.scopus.com/inward/record.url?scp=0042197124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042197124&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0042197124

VL - 72

SP - 321

EP - 323

JO - Horticulture Journal

JF - Horticulture Journal

SN - 2189-0102

IS - 4

ER -