Abstract
Allantoin in fresh tuber of Dioscorea opposita 'Tsukuneimo' was quantitatively analyzed because the compound is thought to prevent inflammation and ulcers in humans as well as to play an important role for storage and translocation of nitrogen in higher plants. The content of ureide (allantoin and allantoic acid) in 'Tsukuneimo' was determined by the modified method of spectrophotometry with phenylhydrazine, whereas the content of allantoin was determined by HPLC. Analyses revealed that 'Tsukuneimo' tubers contained 2.6 mg·gFW-1 of allantoin, but leaves or stems had undetectable levels. D. opposita, 'Yamatoimo' and 'Nagaimo', and D. japonica 'Jinenjo' contained 2.3, 0.47 and 1.2 mg·gFW-1 of allantoin in the tuber, respectively, but negligible amount of allantoic acid in the tuber. Furthermore, Ipomoea batatas and Solanum tuberosum contained less than 0.1 mg·gFW-1 of allantoin and a negligible amount of allantoic acid. Colocasia antiquorum, 'Ishikawawase' and 'Kyouimo' had no detectable amounts of allantoin or allantoic acid.
| Original language | English |
|---|---|
| Pages (from-to) | 321-323 |
| Number of pages | 3 |
| Journal | Journal of the Japanese Society for Horticultural Science |
| Volume | 72 |
| Issue number | 4 |
| Publication status | Published - Jul 2003 |
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Keywords
- Allantoin
- Dioscorea opposita
- Tsukuneimo
- Tuber
- Ureide
ASJC Scopus subject areas
- Horticulture
- Plant Science
Cite this
Quantitative analysis of allantoin in fresh tubers of Dioscorea opposita 'Tsukuneimo'. / Ninomiya, Akio; Murata, Yoshiyuki; Tada, Mikiro; Shimoishi, Yasuaki.
In: Journal of the Japanese Society for Horticultural Science, Vol. 72, No. 4, 07.2003, p. 321-323.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Quantitative analysis of allantoin in fresh tubers of Dioscorea opposita 'Tsukuneimo'
AU - Ninomiya, Akio
AU - Murata, Yoshiyuki
AU - Tada, Mikiro
AU - Shimoishi, Yasuaki
PY - 2003/7
Y1 - 2003/7
N2 - Allantoin in fresh tuber of Dioscorea opposita 'Tsukuneimo' was quantitatively analyzed because the compound is thought to prevent inflammation and ulcers in humans as well as to play an important role for storage and translocation of nitrogen in higher plants. The content of ureide (allantoin and allantoic acid) in 'Tsukuneimo' was determined by the modified method of spectrophotometry with phenylhydrazine, whereas the content of allantoin was determined by HPLC. Analyses revealed that 'Tsukuneimo' tubers contained 2.6 mg·gFW-1 of allantoin, but leaves or stems had undetectable levels. D. opposita, 'Yamatoimo' and 'Nagaimo', and D. japonica 'Jinenjo' contained 2.3, 0.47 and 1.2 mg·gFW-1 of allantoin in the tuber, respectively, but negligible amount of allantoic acid in the tuber. Furthermore, Ipomoea batatas and Solanum tuberosum contained less than 0.1 mg·gFW-1 of allantoin and a negligible amount of allantoic acid. Colocasia antiquorum, 'Ishikawawase' and 'Kyouimo' had no detectable amounts of allantoin or allantoic acid.
AB - Allantoin in fresh tuber of Dioscorea opposita 'Tsukuneimo' was quantitatively analyzed because the compound is thought to prevent inflammation and ulcers in humans as well as to play an important role for storage and translocation of nitrogen in higher plants. The content of ureide (allantoin and allantoic acid) in 'Tsukuneimo' was determined by the modified method of spectrophotometry with phenylhydrazine, whereas the content of allantoin was determined by HPLC. Analyses revealed that 'Tsukuneimo' tubers contained 2.6 mg·gFW-1 of allantoin, but leaves or stems had undetectable levels. D. opposita, 'Yamatoimo' and 'Nagaimo', and D. japonica 'Jinenjo' contained 2.3, 0.47 and 1.2 mg·gFW-1 of allantoin in the tuber, respectively, but negligible amount of allantoic acid in the tuber. Furthermore, Ipomoea batatas and Solanum tuberosum contained less than 0.1 mg·gFW-1 of allantoin and a negligible amount of allantoic acid. Colocasia antiquorum, 'Ishikawawase' and 'Kyouimo' had no detectable amounts of allantoin or allantoic acid.
KW - Allantoin
KW - Dioscorea opposita
KW - Tsukuneimo
KW - Tuber
KW - Ureide
UR - http://www.scopus.com/inward/record.url?scp=0042197124&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042197124&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0042197124
VL - 72
SP - 321
EP - 323
JO - Horticulture Journal
JF - Horticulture Journal
SN - 2189-0102
IS - 4
ER -