Abstract
Abnormal amplification of epidermal growth factor receptor (EGFR) gene has been reported widely in various human tumors. However, the status of amplification of this gene in the process of carcinogenesis is not clearly defined. We used competitive polymerase chain reaction (PCR) to study whether EGFR gene is amplified and the degree of amplification in pre-malignant and malignant oral epithelial lesions, and also examined the relationship between EGFR gene aberration and the development of squamous cell carcinoma (SCC). Genomic DNA was extracted from paraffin sections of 17 cases of oral epithelial dysplasia (ED), four cases of carcinoma in situ (CIS), and 20 cases of untreated primary SCC. The extracted DNA was subjected to competitive PCR to amplify EGFR gene. Amplification of the EGFR gene was observed in three cases (17%) of ED, one case of CIS and four cases (20%) of SCC. In cases showing EGFR gene amplification, the degree of amplification was low in ED and CIS cases, whereas it was extremely high in SCC cases. These results suggest that amplification of EGFR gene occurs in the relatively early stage of the development of oral SCC. However, a high level of EGFR gene accumulation probably plays an important role in the progression to invasive cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 599-604 |
| Number of pages | 6 |
| Journal | Oral Oncology |
| Volume | 37 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- Epidermal growth factor receptor
- Gene amplification
- Neoplastic cell transformation
- Oral cancer
- Polymerase chain reaction
- Squamous cell cancer
ASJC Scopus subject areas
- Oncology
Cite this
Quantitation of epidermal growth factor receptor gene amplification by competitive polymerase chain reaction in pre-malignant and malignant oral epithelial lesions. / Nagatsuka, Hitoshi; Ishiwari, Yuzo; Tsujigiwa, Hidetsugu; Nakano, Keisuke; Nagai, Noriyuki.
In: Oral Oncology, Vol. 37, No. 7, 2001, p. 599-604.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitation of epidermal growth factor receptor gene amplification by competitive polymerase chain reaction in pre-malignant and malignant oral epithelial lesions
AU - Nagatsuka, Hitoshi
AU - Ishiwari, Yuzo
AU - Tsujigiwa, Hidetsugu
AU - Nakano, Keisuke
AU - Nagai, Noriyuki
PY - 2001
Y1 - 2001
N2 - Abnormal amplification of epidermal growth factor receptor (EGFR) gene has been reported widely in various human tumors. However, the status of amplification of this gene in the process of carcinogenesis is not clearly defined. We used competitive polymerase chain reaction (PCR) to study whether EGFR gene is amplified and the degree of amplification in pre-malignant and malignant oral epithelial lesions, and also examined the relationship between EGFR gene aberration and the development of squamous cell carcinoma (SCC). Genomic DNA was extracted from paraffin sections of 17 cases of oral epithelial dysplasia (ED), four cases of carcinoma in situ (CIS), and 20 cases of untreated primary SCC. The extracted DNA was subjected to competitive PCR to amplify EGFR gene. Amplification of the EGFR gene was observed in three cases (17%) of ED, one case of CIS and four cases (20%) of SCC. In cases showing EGFR gene amplification, the degree of amplification was low in ED and CIS cases, whereas it was extremely high in SCC cases. These results suggest that amplification of EGFR gene occurs in the relatively early stage of the development of oral SCC. However, a high level of EGFR gene accumulation probably plays an important role in the progression to invasive cancer.
AB - Abnormal amplification of epidermal growth factor receptor (EGFR) gene has been reported widely in various human tumors. However, the status of amplification of this gene in the process of carcinogenesis is not clearly defined. We used competitive polymerase chain reaction (PCR) to study whether EGFR gene is amplified and the degree of amplification in pre-malignant and malignant oral epithelial lesions, and also examined the relationship between EGFR gene aberration and the development of squamous cell carcinoma (SCC). Genomic DNA was extracted from paraffin sections of 17 cases of oral epithelial dysplasia (ED), four cases of carcinoma in situ (CIS), and 20 cases of untreated primary SCC. The extracted DNA was subjected to competitive PCR to amplify EGFR gene. Amplification of the EGFR gene was observed in three cases (17%) of ED, one case of CIS and four cases (20%) of SCC. In cases showing EGFR gene amplification, the degree of amplification was low in ED and CIS cases, whereas it was extremely high in SCC cases. These results suggest that amplification of EGFR gene occurs in the relatively early stage of the development of oral SCC. However, a high level of EGFR gene accumulation probably plays an important role in the progression to invasive cancer.
KW - Epidermal growth factor receptor
KW - Gene amplification
KW - Neoplastic cell transformation
KW - Oral cancer
KW - Polymerase chain reaction
KW - Squamous cell cancer
UR - http://www.scopus.com/inward/record.url?scp=0034833599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034833599&partnerID=8YFLogxK
U2 - 10.1016/S1368-8375(01)00051-3
DO - 10.1016/S1368-8375(01)00051-3
M3 - Article
C2 - 11564582
AN - SCOPUS:0034833599
VL - 37
SP - 599
EP - 604
JO - Oral Oncology
JF - Oral Oncology
SN - 1368-8375
IS - 7
ER -