Quantification of TECTA and DFNA5 expression in the developing mouse cochlea

Y. Maeda, K. Fukushima, N. Kasai, M. Maeta, K. Nishizaki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

TECTA and DFNA5 are the mouse orthologues of the human deafness-associated genes TECTA and DFNA5. To determine how expression of these genes is regulated during development, relative mRNA abundance was examined in mice by non-radioactive RT-PCR. TECTA mRNA was detected on embryonic day 15 (E15), increased to its highest level on postnatal day 3 (P3) and then dramatically decreased by P15. Low levels persisted (adulthood, P45 to 67) with mean mRNA abundance after P15 less than 25% of P3 levels. DFNA5 mRNA expression was constant throughout these time points. These results imply that TECTA is transcribed at a particularly high level during tectorial membrane morphogenesis. In contrast, DFNA5 is present in both the developing and mature cochlea.

Original languageEnglish
Pages (from-to)3223-3226
Number of pages4
JournalNeuroReport
Volume12
Issue number15
DOIs
Publication statusPublished - Oct 29 2001

Keywords

  • Cochlear development
  • DFNA5
  • Deafness-associated genes
  • Non-radioactive RT-PCR
  • TECTA

ASJC Scopus subject areas

  • Neuroscience(all)

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