Quality control of photosystem II: Thylakoid unstacking necessary to avoid further damage to the D1 protein and to facilitate D1 degradation under light stress in spinach thylakoids

Mahbuba Khatoon, Kayo Inagawa, Pavel Pospišil, Amu Yamashita, Miho Nishimura, Björn Lundin, Junko Horie, Noriko Morita, Anjana Jajoo, Yoko Yamamoto, Yasusi Yamamoto

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Photosystem II is vulnerable to light damage. The reaction center-binding D1 protein is impaired during excessive illumination and is degraded and removed from photosystem II. Using isolated spinach thylakoids, we investigated the relationship between light-induced unstacking of thylakoids and damage to the D1 protein. Under light stress, thylakoids were expected to become unstacked so that the photodamaged photosystem II complexes in the grana and the proteases could move on the thylakoids for repair. Excessive light induced irreversible unstacking of thylakoids. By comparing the effects of light stress on stacked and unstacked thylakoids, photoinhibition of photosystem II was found to be more prominent in stacked thylakoids than in unstacked thylakoids. In accordance with this finding, EPR spin trapping measurements demonstrated higher production of hydroxyl radicals in stacked thylakoids than in unstacked thylakoids. We propose that unstacking of thylakoids has a crucial role in avoiding further damage to the D1 protein and facilitating degradation of the photodamaged D1 protein under light stress.

Original languageEnglish
Pages (from-to)25343-25352
Number of pages10
JournalJournal of Biological Chemistry
Volume284
Issue number37
DOIs
Publication statusPublished - Sep 11 2009

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Thylakoids
Photosystem II Protein Complex
Spinacia oleracea
Quality Control
Quality control
Light
Degradation
Proteins
Hydroxyl Radical
Paramagnetic resonance
Repair
Peptide Hydrolases
Lighting
Spin Trapping
Proteolysis
Carrier Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Quality control of photosystem II : Thylakoid unstacking necessary to avoid further damage to the D1 protein and to facilitate D1 degradation under light stress in spinach thylakoids. / Khatoon, Mahbuba; Inagawa, Kayo; Pospišil, Pavel; Yamashita, Amu; Nishimura, Miho; Lundin, Björn; Horie, Junko; Morita, Noriko; Jajoo, Anjana; Yamamoto, Yoko; Yamamoto, Yasusi.

In: Journal of Biological Chemistry, Vol. 284, No. 37, 11.09.2009, p. 25343-25352.

Research output: Contribution to journalArticle

Khatoon, Mahbuba ; Inagawa, Kayo ; Pospišil, Pavel ; Yamashita, Amu ; Nishimura, Miho ; Lundin, Björn ; Horie, Junko ; Morita, Noriko ; Jajoo, Anjana ; Yamamoto, Yoko ; Yamamoto, Yasusi. / Quality control of photosystem II : Thylakoid unstacking necessary to avoid further damage to the D1 protein and to facilitate D1 degradation under light stress in spinach thylakoids. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 37. pp. 25343-25352.
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