Quality control of photosystem II: Reactive oxygen species are responsible for the damage to photosystem II under moderate heat stress

Amu Yamashita, Nobuyoshi Nijo, Pavel Pospíšil, Noriko Morita, Daichi Takenaka, Ryota Aminaka, Yoko Yamamoto, Yasusi Yamamoto

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Moderate heat stress (40°C for 30 min) on spinach thylakoid membranes induced cleavage of the reaction center-binding D1 protein of photosystem II, aggregation of the D1 protein with the neighboring polypeptides D2 and CP43, and release of three extrinsic proteins, PsbO, -P, and -Q. These heat-induced events were suppressed under anaerobic conditions or by the addition of sodium ascorbate, a general scavenger of reactive oxygen species. In accordance with this, singlet oxygen and hydroxyl radicals were detected in spinach photosystem II membranes incubated at 40°C for 30 min with electron paramagnetic resonance spin-trapping spectroscopy. The moderate heat stress also induced significant lipid peroxidation under aerobic conditions. We suggest that the reactive oxygen species are generated by heat-induced inactivation of a water-oxidizing manganese complex and through lipid peroxidation. Although occurring in the dark, the damages caused by the moderate heat stress to photosystem II are quite similar to those induced by excessive illumination where reactive oxygen species are involved.

Original languageEnglish
Pages (from-to)28380-28391
Number of pages12
JournalJournal of Biological Chemistry
Volume283
Issue number42
DOIs
Publication statusPublished - Oct 17 2008

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Yamashita, A., Nijo, N., Pospíšil, P., Morita, N., Takenaka, D., Aminaka, R., Yamamoto, Y., & Yamamoto, Y. (2008). Quality control of photosystem II: Reactive oxygen species are responsible for the damage to photosystem II under moderate heat stress. Journal of Biological Chemistry, 283(42), 28380-28391. https://doi.org/10.1074/jbc.M710465200