Quality Control of Photosystem II: Lipid Peroxidation Accelerates Photoinhibition under Excessive Illumination

Tiffanie Chan, Yurika Shimizu, Pavel Pospíšil, Nobuyoshi Nijo, Anna Fujiwara, Yoshito Taninaka, Tomomi Ishikawa, Haruka Hori, Daisuke Nanba, Aya Imai, Noriko Morita, Miho Nishimura, Yohei Izumi, Yoko Yamamoto, Hideki Kobayashi, Naoki Mizusawa, Hajime Wada, Yasusi Yamamoto

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Environmental stresses lower the efficiency of photosynthesis and sometimes cause irreversible damage to plant functions. When spinach thylakoids and Photosystem II membranes were illuminated with excessive visible light (100-1,000 μmol photons m-1 s-1) for 10 min at either 20°C or 30°C, the optimum quantum yield of Photosystem II decreased as the light intensity and temperature increased. Reactive oxygen species and endogenous cationic radicals produced through a photochemical reaction at and/or near the reaction center have been implicated in the damage to the D1 protein. Here we present evidence that lipid peroxidation induced by the illumination is involved in the damage to the D1 protein and the subunits of the light-harvesting complex of Photosystem II. This is reasoned from the results that considerable lipid peroxidation occurred in the thylakoids in the light, and that lipoxygenase externally added in the dark induced inhibition of Photosystem II activity in the thylakoids, production of singlet oxygen, which was monitored by electron paramagnetic resonance spin trapping, and damage to the D1 protein, in parallel with lipid peroxidation. Modification of the subunits of the light-harvesting complex of Photosystem II by malondialdehyde as well as oxidation of the subunits was also observed. We suggest that mainly singlet oxygen formed through lipid peroxidation under light stress participates in damaging the Photosystem II subunits.

Original languageEnglish
Article numbere52100
JournalPLoS One
Volume7
Issue number12
DOIs
Publication statusPublished - Dec 27 2012

Fingerprint

Photosystem II Protein Complex
photoinhibition
Lighting
Quality Control
photosystem II
Lipid Peroxidation
quality control
Quality control
lighting
lipid peroxidation
Lipids
Light
D1 protein
Thylakoids
thylakoids
Singlet Oxygen
singlet oxygen
light harvesting complex
photochemical reactions
Spin Trapping

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chan, T., Shimizu, Y., Pospíšil, P., Nijo, N., Fujiwara, A., Taninaka, Y., ... Yamamoto, Y. (2012). Quality Control of Photosystem II: Lipid Peroxidation Accelerates Photoinhibition under Excessive Illumination. PLoS One, 7(12), [e52100]. https://doi.org/10.1371/journal.pone.0052100

Quality Control of Photosystem II : Lipid Peroxidation Accelerates Photoinhibition under Excessive Illumination. / Chan, Tiffanie; Shimizu, Yurika; Pospíšil, Pavel; Nijo, Nobuyoshi; Fujiwara, Anna; Taninaka, Yoshito; Ishikawa, Tomomi; Hori, Haruka; Nanba, Daisuke; Imai, Aya; Morita, Noriko; Nishimura, Miho; Izumi, Yohei; Yamamoto, Yoko; Kobayashi, Hideki; Mizusawa, Naoki; Wada, Hajime; Yamamoto, Yasusi.

In: PLoS One, Vol. 7, No. 12, e52100, 27.12.2012.

Research output: Contribution to journalArticle

Chan, T, Shimizu, Y, Pospíšil, P, Nijo, N, Fujiwara, A, Taninaka, Y, Ishikawa, T, Hori, H, Nanba, D, Imai, A, Morita, N, Nishimura, M, Izumi, Y, Yamamoto, Y, Kobayashi, H, Mizusawa, N, Wada, H & Yamamoto, Y 2012, 'Quality Control of Photosystem II: Lipid Peroxidation Accelerates Photoinhibition under Excessive Illumination', PLoS One, vol. 7, no. 12, e52100. https://doi.org/10.1371/journal.pone.0052100
Chan, Tiffanie ; Shimizu, Yurika ; Pospíšil, Pavel ; Nijo, Nobuyoshi ; Fujiwara, Anna ; Taninaka, Yoshito ; Ishikawa, Tomomi ; Hori, Haruka ; Nanba, Daisuke ; Imai, Aya ; Morita, Noriko ; Nishimura, Miho ; Izumi, Yohei ; Yamamoto, Yoko ; Kobayashi, Hideki ; Mizusawa, Naoki ; Wada, Hajime ; Yamamoto, Yasusi. / Quality Control of Photosystem II : Lipid Peroxidation Accelerates Photoinhibition under Excessive Illumination. In: PLoS One. 2012 ; Vol. 7, No. 12.
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