Quality control of photosystem II FtsH hexamers are localized near Photosystem II at grana for the swift repair of damage

Miho Nishimura, Yosuke Nakayama, Mari Yoshida, Kensuke Ohashi, Noriko Morita, Hideki Kobayashi, Yasusi Yamamoto

Research output: Contribution to journalArticle

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Abstract

The reaction center-binding D1 protein of Photosystem II is oxidatively damaged by excessive visible light or moderate heat stress. The metalloprotease FtsH has been suggested as responsible for the degradation of the D1 protein. We have analyzed the distribution and subunit structures of FtsH in spinach thylakoids and various membrane fractions derived from the thylakoids using clear native polyacrylamide gel electrophoresis and Western blot analysis. FtsH was found not only in the stroma thylakoids but also in the Photosystem IIenriched grana membranes. Monomeric, dimeric, and hexameric FtsH proteases were present as major subunit structures in thylakoids, whereas only hexameric FtsH proteases were detected in Triton X-100-solubilized Photosystem II membranes. Importantly, among the membrane fractions examined, hexameric FtsH proteases were most abundant in the Photosystem II membranes. In accordance with this finding, D1 degradation took place in the Photosystem II membranes under light stress. Sucrose density gradient centrifugation analysis of thylakoids and the Photosystem II membranes solubilized with n-dodecyl-β-D-maltoside and a chemical crosslinking study of thylakoids showed localization of FtsH near the Photosystem II light-harvesting chlorophyll-protein supercomplexes in the grana. These results suggest that part of the FtsH hexamers are juxtapositioned to PSII complexes in the grana in darkness, carrying out immediate degradation of the photodamaged D1 protein under light stress.

Original languageEnglish
Pages (from-to)41972-41981
Number of pages10
JournalJournal of Biological Chemistry
Volume285
Issue number53
DOIs
Publication statusPublished - Dec 31 2010

Fingerprint

Thylakoids
Photosystem II Protein Complex
Quality Control
Quality control
Repair
Membranes
Peptide Hydrolases
Degradation
Light-Harvesting Protein Complexes
Light
Centrifugation
Octoxynol
Metalloproteases
Native Polyacrylamide Gel Electrophoresis
Electrophoresis
Crosslinking
Spinacia oleracea
Density Gradient Centrifugation
Sucrose
Darkness

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Quality control of photosystem II FtsH hexamers are localized near Photosystem II at grana for the swift repair of damage. / Nishimura, Miho; Nakayama, Yosuke; Yoshida, Mari; Ohashi, Kensuke; Morita, Noriko; Kobayashi, Hideki; Yamamoto, Yasusi.

In: Journal of Biological Chemistry, Vol. 285, No. 53, 31.12.2010, p. 41972-41981.

Research output: Contribution to journalArticle

Nishimura, Miho ; Nakayama, Yosuke ; Yoshida, Mari ; Ohashi, Kensuke ; Morita, Noriko ; Kobayashi, Hideki ; Yamamoto, Yasusi. / Quality control of photosystem II FtsH hexamers are localized near Photosystem II at grana for the swift repair of damage. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 53. pp. 41972-41981.
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