QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley

Asuka Kodama, Ryouhei Narita, Makoto Yamaguchi, Hiroshi Hisano, Shunsuke Adachi, Hiroki Takagi, Taiichiro Ookawa, Kazuhiro Sato, Tadashi Hirasawa

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Enhancing salt stress tolerance is a key strategy for increasing global food production. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley (Hordeum vulgare) accession, ‘OUC613’, but not in the salt-tolerant accession, ‘OUE812’, resulting in large differences in grain yield. Here, we examined the underlying causes of the difference in grain fertility between these accessions under long-term treatment with 150 or 200 mM NaCl from the seedling stage to harvest and identified quantitative trait loci (QTLs) for maintaining grain fertility. In an artificial pollination experiment of the two accessions, grain fertility was significantly reduced only in OUC613 plants produced using pollen from plants grown under NaCl stress, suggesting that the low grain fertility of OUC613 was mainly due to reduced pollen fertility. Using QTL-seq combined with exome-capture sequencing and composite interval mapping of recombinant inbred lines derived from a cross between OUE812 and OUC613, we identified a QTL (qRP-2Hb) for grain fertility on chromosome 2H. The QTL region includes two genes encoding an F-box protein and a TIFY protein that are associated with male sterility, highlighting the importance of this region for maintaining grain fertility under salt stress.

    Original languageEnglish
    Pages (from-to)561-570
    Number of pages10
    JournalBreeding Science
    Volume68
    Issue number5
    DOIs
    Publication statusPublished - 2018

    Keywords

    • Composite interval mapping
    • Exome QTL-seq
    • Grain fertility
    • Hordeum vulgare
    • Pollen sterility
    • Quantitative trait locus
    • Salt stress

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Genetics
    • Plant Science

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