QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley

Asuka Kodama, Ryouhei Narita, Makoto Yamaguchi, Hiroshi Hisano, Shunsuke Adachi, Hiroki Takagi, Taiichiro Ookawa, Kazuhiro Sato, Tadashi Hirasawa

Research output: Contribution to journalArticle

Abstract

Enhancing salt stress tolerance is a key strategy for increasing global food production. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley (Hordeum vulgare) accession, ‘OUC613’, but not in the salt-tolerant accession, ‘OUE812’, resulting in large differences in grain yield. Here, we examined the underlying causes of the difference in grain fertility between these accessions under long-term treatment with 150 or 200 mM NaCl from the seedling stage to harvest and identified quantitative trait loci (QTLs) for maintaining grain fertility. In an artificial pollination experiment of the two accessions, grain fertility was significantly reduced only in OUC613 plants produced using pollen from plants grown under NaCl stress, suggesting that the low grain fertility of OUC613 was mainly due to reduced pollen fertility. Using QTL-seq combined with exome-capture sequencing and composite interval mapping of recombinant inbred lines derived from a cross between OUE812 and OUC613, we identified a QTL (qRP-2Hb) for grain fertility on chromosome 2H. The QTL region includes two genes encoding an F-box protein and a TIFY protein that are associated with male sterility, highlighting the importance of this region for maintaining grain fertility under salt stress.

Original languageEnglish
Pages (from-to)561-570
Number of pages10
JournalBreeding Science
Volume68
Issue number5
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Exome
Quantitative Trait Loci
Hordeum
salt stress
Fertility
quantitative trait loci
Salts
barley
Pollen
pollen
salts
F-box proteins
F-Box Proteins
Salt-Tolerance
male fertility
Pollination
Male Infertility
food production
Salinity
stress tolerance

Keywords

  • Composite interval mapping
  • Exome QTL-seq
  • Grain fertility
  • Hordeum vulgare
  • Pollen sterility
  • Quantitative trait locus
  • Salt stress

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley. / Kodama, Asuka; Narita, Ryouhei; Yamaguchi, Makoto; Hisano, Hiroshi; Adachi, Shunsuke; Takagi, Hiroki; Ookawa, Taiichiro; Sato, Kazuhiro; Hirasawa, Tadashi.

In: Breeding Science, Vol. 68, No. 5, 01.01.2018, p. 561-570.

Research output: Contribution to journalArticle

Kodama, A, Narita, R, Yamaguchi, M, Hisano, H, Adachi, S, Takagi, H, Ookawa, T, Sato, K & Hirasawa, T 2018, 'QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley', Breeding Science, vol. 68, no. 5, pp. 561-570. https://doi.org/10.1270/jsbbs.18082
Kodama, Asuka ; Narita, Ryouhei ; Yamaguchi, Makoto ; Hisano, Hiroshi ; Adachi, Shunsuke ; Takagi, Hiroki ; Ookawa, Taiichiro ; Sato, Kazuhiro ; Hirasawa, Tadashi. / QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley. In: Breeding Science. 2018 ; Vol. 68, No. 5. pp. 561-570.
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