Abstract
Enhancing salt stress tolerance is a key strategy for increasing global food production. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley (Hordeum vulgare) accession, ‘OUC613’, but not in the salt-tolerant accession, ‘OUE812’, resulting in large differences in grain yield. Here, we examined the underlying causes of the difference in grain fertility between these accessions under long-term treatment with 150 or 200 mM NaCl from the seedling stage to harvest and identified quantitative trait loci (QTLs) for maintaining grain fertility. In an artificial pollination experiment of the two accessions, grain fertility was significantly reduced only in OUC613 plants produced using pollen from plants grown under NaCl stress, suggesting that the low grain fertility of OUC613 was mainly due to reduced pollen fertility. Using QTL-seq combined with exome-capture sequencing and composite interval mapping of recombinant inbred lines derived from a cross between OUE812 and OUC613, we identified a QTL (qRP-2Hb) for grain fertility on chromosome 2H. The QTL region includes two genes encoding an F-box protein and a TIFY protein that are associated with male sterility, highlighting the importance of this region for maintaining grain fertility under salt stress.
| Original language | English |
|---|---|
| Pages (from-to) | 561-570 |
| Number of pages | 10 |
| Journal | Breeding Science |
| Volume | 68 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jan 1 2018 |
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Keywords
- Composite interval mapping
- Exome QTL-seq
- Grain fertility
- Hordeum vulgare
- Pollen sterility
- Quantitative trait locus
- Salt stress
ASJC Scopus subject areas
- Agronomy and Crop Science
- Genetics
- Plant Science
Cite this
QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley. / Kodama, Asuka; Narita, Ryouhei; Yamaguchi, Makoto; Hisano, Hiroshi; Adachi, Shunsuke; Takagi, Hiroki; Ookawa, Taiichiro; Sato, Kazuhiro; Hirasawa, Tadashi.
In: Breeding Science, Vol. 68, No. 5, 01.01.2018, p. 561-570.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley
AU - Kodama, Asuka
AU - Narita, Ryouhei
AU - Yamaguchi, Makoto
AU - Hisano, Hiroshi
AU - Adachi, Shunsuke
AU - Takagi, Hiroki
AU - Ookawa, Taiichiro
AU - Sato, Kazuhiro
AU - Hirasawa, Tadashi
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Enhancing salt stress tolerance is a key strategy for increasing global food production. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley (Hordeum vulgare) accession, ‘OUC613’, but not in the salt-tolerant accession, ‘OUE812’, resulting in large differences in grain yield. Here, we examined the underlying causes of the difference in grain fertility between these accessions under long-term treatment with 150 or 200 mM NaCl from the seedling stage to harvest and identified quantitative trait loci (QTLs) for maintaining grain fertility. In an artificial pollination experiment of the two accessions, grain fertility was significantly reduced only in OUC613 plants produced using pollen from plants grown under NaCl stress, suggesting that the low grain fertility of OUC613 was mainly due to reduced pollen fertility. Using QTL-seq combined with exome-capture sequencing and composite interval mapping of recombinant inbred lines derived from a cross between OUE812 and OUC613, we identified a QTL (qRP-2Hb) for grain fertility on chromosome 2H. The QTL region includes two genes encoding an F-box protein and a TIFY protein that are associated with male sterility, highlighting the importance of this region for maintaining grain fertility under salt stress.
AB - Enhancing salt stress tolerance is a key strategy for increasing global food production. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley (Hordeum vulgare) accession, ‘OUC613’, but not in the salt-tolerant accession, ‘OUE812’, resulting in large differences in grain yield. Here, we examined the underlying causes of the difference in grain fertility between these accessions under long-term treatment with 150 or 200 mM NaCl from the seedling stage to harvest and identified quantitative trait loci (QTLs) for maintaining grain fertility. In an artificial pollination experiment of the two accessions, grain fertility was significantly reduced only in OUC613 plants produced using pollen from plants grown under NaCl stress, suggesting that the low grain fertility of OUC613 was mainly due to reduced pollen fertility. Using QTL-seq combined with exome-capture sequencing and composite interval mapping of recombinant inbred lines derived from a cross between OUE812 and OUC613, we identified a QTL (qRP-2Hb) for grain fertility on chromosome 2H. The QTL region includes two genes encoding an F-box protein and a TIFY protein that are associated with male sterility, highlighting the importance of this region for maintaining grain fertility under salt stress.
KW - Composite interval mapping
KW - Exome QTL-seq
KW - Grain fertility
KW - Hordeum vulgare
KW - Pollen sterility
KW - Quantitative trait locus
KW - Salt stress
UR - http://www.scopus.com/inward/record.url?scp=85059339591&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85059339591&partnerID=8YFLogxK
U2 - 10.1270/jsbbs.18082
DO - 10.1270/jsbbs.18082
M3 - Article
AN - SCOPUS:85059339591
VL - 68
SP - 561
EP - 570
JO - Breeding Science
JF - Breeding Science
SN - 1344-7610
IS - 5
ER -