QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups

L. A. Marquez-Cedillo, P. M. Hayes, B. L. Jones, A. Kleinhofs, W. G. Legge, B. C. Rossnagel, Kazuhiro Sato, E. Ullrich, D. M. Wesenberg

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Characterization of the determinants of economically important phenotypes showing complex inheritance should lead to the more effective use of genetic resources. This study was conducted to determine the number, genome location and effects of QTLs determining malting quality in the two North American barley quality standards. Using a doubled-haploid population of 140 lines from the cross of HarringtonxMorex, malting quality phenotype data sets from eight environments, and a 107-marker linkage map, QTL analyses were performed using simple interval mapping and simplified composite interval mapping procedures. Seventeen QTLs were associated with seven grain and malting quality traits (percentage of plump kernels, test weight, grain protein percentage, soluble/total protein ratio, α-amylase activity, diastatic power and malt-extract percentage). QTLs for multiple traits were coincident. The loci controlling inflorescence type [vrs1 on chromosome 2(2H) and int-c on chromosome 4(4H)] were coincident with QTLs affecting all traits except malt-extract percentage. The largest effect QTLs, for the percentage of plump kernels, test weight protein percentage, S/T ratio and diastatic power, were coincident with the vrs1 locus. QTL analyses were conducted separately for each sub-population (six-rowed and two-rowed). Eleven new QTLs were detected in the subpopulations. There were significant interactions between the vrs1 and int-c loci for grain-protein percentage and S/T protein ratio. Results suggest that this mating of two different germplasm groups caused a disruption of the balance of traits. Information on the number, position and effects of QTLs determining components of malting quality may be useful for maintaining specific allele configurations that determine target quality profiles.

Original languageEnglish
Pages (from-to)173-184
Number of pages12
JournalTheoretical And Applied Genetics
Volume101
Issue number1-2
DOIs
Publication statusPublished - 2000

Fingerprint

malting quality
Haploidy
doubled haploids
Hordeum
quantitative trait loci
germplasm
Protein S
barley
Phenotype
Weights and Measures
Inflorescence
Chromosomes, Human, Pair 4
Proteins
Chromosomes, Human, Pair 2
Amylases
Population
malt extract
Alleles
grain protein
Genome

Keywords

  • Hordeum vulgare
  • Quality traits
  • Quantitative trait loci
  • Six-rowed
  • Two-rowed

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Marquez-Cedillo, L. A., Hayes, P. M., Jones, B. L., Kleinhofs, A., Legge, W. G., Rossnagel, B. C., ... Wesenberg, D. M. (2000). QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups. Theoretical And Applied Genetics, 101(1-2), 173-184. https://doi.org/10.1007/s001220051466

QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups. / Marquez-Cedillo, L. A.; Hayes, P. M.; Jones, B. L.; Kleinhofs, A.; Legge, W. G.; Rossnagel, B. C.; Sato, Kazuhiro; Ullrich, E.; Wesenberg, D. M.

In: Theoretical And Applied Genetics, Vol. 101, No. 1-2, 2000, p. 173-184.

Research output: Contribution to journalArticle

Marquez-Cedillo, L. A. ; Hayes, P. M. ; Jones, B. L. ; Kleinhofs, A. ; Legge, W. G. ; Rossnagel, B. C. ; Sato, Kazuhiro ; Ullrich, E. ; Wesenberg, D. M. / QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups. In: Theoretical And Applied Genetics. 2000 ; Vol. 101, No. 1-2. pp. 173-184.
@article{792fa7f4798549b79e35b064e5b8265c,
title = "QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups",
abstract = "Characterization of the determinants of economically important phenotypes showing complex inheritance should lead to the more effective use of genetic resources. This study was conducted to determine the number, genome location and effects of QTLs determining malting quality in the two North American barley quality standards. Using a doubled-haploid population of 140 lines from the cross of HarringtonxMorex, malting quality phenotype data sets from eight environments, and a 107-marker linkage map, QTL analyses were performed using simple interval mapping and simplified composite interval mapping procedures. Seventeen QTLs were associated with seven grain and malting quality traits (percentage of plump kernels, test weight, grain protein percentage, soluble/total protein ratio, α-amylase activity, diastatic power and malt-extract percentage). QTLs for multiple traits were coincident. The loci controlling inflorescence type [vrs1 on chromosome 2(2H) and int-c on chromosome 4(4H)] were coincident with QTLs affecting all traits except malt-extract percentage. The largest effect QTLs, for the percentage of plump kernels, test weight protein percentage, S/T ratio and diastatic power, were coincident with the vrs1 locus. QTL analyses were conducted separately for each sub-population (six-rowed and two-rowed). Eleven new QTLs were detected in the subpopulations. There were significant interactions between the vrs1 and int-c loci for grain-protein percentage and S/T protein ratio. Results suggest that this mating of two different germplasm groups caused a disruption of the balance of traits. Information on the number, position and effects of QTLs determining components of malting quality may be useful for maintaining specific allele configurations that determine target quality profiles.",
keywords = "Hordeum vulgare, Quality traits, Quantitative trait loci, Six-rowed, Two-rowed",
author = "Marquez-Cedillo, {L. A.} and Hayes, {P. M.} and Jones, {B. L.} and A. Kleinhofs and Legge, {W. G.} and Rossnagel, {B. C.} and Kazuhiro Sato and E. Ullrich and Wesenberg, {D. M.}",
year = "2000",
doi = "10.1007/s001220051466",
language = "English",
volume = "101",
pages = "173--184",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "1-2",

}

TY - JOUR

T1 - QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups

AU - Marquez-Cedillo, L. A.

AU - Hayes, P. M.

AU - Jones, B. L.

AU - Kleinhofs, A.

AU - Legge, W. G.

AU - Rossnagel, B. C.

AU - Sato, Kazuhiro

AU - Ullrich, E.

AU - Wesenberg, D. M.

PY - 2000

Y1 - 2000

N2 - Characterization of the determinants of economically important phenotypes showing complex inheritance should lead to the more effective use of genetic resources. This study was conducted to determine the number, genome location and effects of QTLs determining malting quality in the two North American barley quality standards. Using a doubled-haploid population of 140 lines from the cross of HarringtonxMorex, malting quality phenotype data sets from eight environments, and a 107-marker linkage map, QTL analyses were performed using simple interval mapping and simplified composite interval mapping procedures. Seventeen QTLs were associated with seven grain and malting quality traits (percentage of plump kernels, test weight, grain protein percentage, soluble/total protein ratio, α-amylase activity, diastatic power and malt-extract percentage). QTLs for multiple traits were coincident. The loci controlling inflorescence type [vrs1 on chromosome 2(2H) and int-c on chromosome 4(4H)] were coincident with QTLs affecting all traits except malt-extract percentage. The largest effect QTLs, for the percentage of plump kernels, test weight protein percentage, S/T ratio and diastatic power, were coincident with the vrs1 locus. QTL analyses were conducted separately for each sub-population (six-rowed and two-rowed). Eleven new QTLs were detected in the subpopulations. There were significant interactions between the vrs1 and int-c loci for grain-protein percentage and S/T protein ratio. Results suggest that this mating of two different germplasm groups caused a disruption of the balance of traits. Information on the number, position and effects of QTLs determining components of malting quality may be useful for maintaining specific allele configurations that determine target quality profiles.

AB - Characterization of the determinants of economically important phenotypes showing complex inheritance should lead to the more effective use of genetic resources. This study was conducted to determine the number, genome location and effects of QTLs determining malting quality in the two North American barley quality standards. Using a doubled-haploid population of 140 lines from the cross of HarringtonxMorex, malting quality phenotype data sets from eight environments, and a 107-marker linkage map, QTL analyses were performed using simple interval mapping and simplified composite interval mapping procedures. Seventeen QTLs were associated with seven grain and malting quality traits (percentage of plump kernels, test weight, grain protein percentage, soluble/total protein ratio, α-amylase activity, diastatic power and malt-extract percentage). QTLs for multiple traits were coincident. The loci controlling inflorescence type [vrs1 on chromosome 2(2H) and int-c on chromosome 4(4H)] were coincident with QTLs affecting all traits except malt-extract percentage. The largest effect QTLs, for the percentage of plump kernels, test weight protein percentage, S/T ratio and diastatic power, were coincident with the vrs1 locus. QTL analyses were conducted separately for each sub-population (six-rowed and two-rowed). Eleven new QTLs were detected in the subpopulations. There were significant interactions between the vrs1 and int-c loci for grain-protein percentage and S/T protein ratio. Results suggest that this mating of two different germplasm groups caused a disruption of the balance of traits. Information on the number, position and effects of QTLs determining components of malting quality may be useful for maintaining specific allele configurations that determine target quality profiles.

KW - Hordeum vulgare

KW - Quality traits

KW - Quantitative trait loci

KW - Six-rowed

KW - Two-rowed

UR - http://www.scopus.com/inward/record.url?scp=0033928015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033928015&partnerID=8YFLogxK

U2 - 10.1007/s001220051466

DO - 10.1007/s001220051466

M3 - Article

AN - SCOPUS:0033928015

VL - 101

SP - 173

EP - 184

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 1-2

ER -