QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley

K. Hori, T. Kobayashi, Kazuhiro Sato, K. Takeda

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Fusarium head blight (FHB) resistance was evaluated in a set of recombinant inbred (RI) lines from a cross between Russia 6 (resistant) and H.E.S. 4 (susceptible), which had one of the widest differences of FHB resistance reactions among ca. 5,000 barley germplasm accessions in Okayama University. Field-grown spikes were sampled and inoculated by the 'cut-spike test'. Resistance reactions on the parents and RI lines were scored by eleven grades, from resistant (0) to susceptible (10). Quantitative trait loci (QTL) analysis detected three QTL: two located on the long arm of chromosome 2H, and another on the short arm of chromosome 5H. A QTL located on chromosome 2H was coincident with the vrs1 locus, which governs inflorescence row type. The other QTL on chromosome 2H was positioned in the vicinity of cleistogamy locus (cly1 or Cly2) that determines inflorescence opening/closing. Resistant gene analog (RGA) and expressed sequence tag (EST) markers with homology for disease resistance genes were integrated into the high-density linkage map. Most of these markers were not localized near the identified resistance QTL, except for one RGA marker (FXLRRfor_XLRRrev170) localized in the vicinity of the cly1/Cly2 locus. Five AFLP markers localized in the vicinity of the identified QTL were sequenced to convert them into sequence tagged site (STS) markers. Genotyping of each RI line using two AFLP-STS markers and the vrs1 locus indicated that the RI lines with three Russia 6 QTL alleles exhibited the same level of high FHB resistance reactions as Russia 6. In contrast, RI lines with three susceptible alleles showed reactions close to H.E.S. 4. Therefore, the markers closely linked to the QTL can be efficiently used for the selection of resistance.

Original languageEnglish
Pages (from-to)1661-1672
Number of pages12
JournalTheoretical And Applied Genetics
Volume111
Issue number8
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Fusarium head blight
Quantitative Trait Loci
Fusarium
Hordeum
chromosome mapping
quantitative trait loci
barley
inbred lines
Russia
inflorescences
Chromosomes
Sequence Tagged Sites
chromosomes
Inflorescence
loci
amplified fragment length polymorphism
Alleles
cleistogamy
Genes
alleles

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley. / Hori, K.; Kobayashi, T.; Sato, Kazuhiro; Takeda, K.

In: Theoretical And Applied Genetics, Vol. 111, No. 8, 11.2005, p. 1661-1672.

Research output: Contribution to journalArticle

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