QTL analysis of agronomic traits in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups

L. A. Marquez-Cedillo, P. M. Hayes, A. Kleinhofs, W. G. Legge, B. G. Rossnagel, K. Sato, S. E. Ullrich, D. M. Wesenberg

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

A better understanding of the genetics of complex traits, such as yield, may be achieved by using molecular tools. This study was conducted to estimate the number, genome location, effect and allele phase of QTLs determining agronomic traits in the two North American malting barley (Hordeum vulgare L.) quality variety standards. Using a doubled haploid population of 140 lines from the cross of two-rowed Harrington×six-rowed Morex, agronomic phenotypic data sets from nine environments, and a 107-marker linkage map, we performed QTL analyses using simple interval mapping and simplified composite interval mapping procedures. Thirty-five QTLs were associated, either across environments or in individual environments, with five grain and agronomic traits (yield, kernel plumpness, test weight, heading date, and plant height). Significant QTL×environment interaction was detected for all traits. These interactions resulted from both changes in the magnitude of response and changes in the sign of the allelic effect. QTLs for multiple traits were coincident. The vrs1 locus on chromosome 2 (2H), which determines inflorescence row type, was coincident with the largest-effect QTL determining four traits (yield, kernel plumpness, test weight, and plant height). QTL analyses were also conducted separately for each sub-population (six-rowed and two-rowed). Seven new QTLs were detected in the sub-populations. Positive transgressive segregants were found for all traits, but they were more prevalent in the six-rowed sub-population. QTL analysis should be useful for identifying candidate genes and introgressing favorable alleles between germplasm groups.

Original languageEnglish
Pages (from-to)625-637
Number of pages13
JournalTheoretical and Applied Genetics
Volume103
Issue number4
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • Agronomic traits
  • Hordeum vulgare
  • Quantitative trait loci
  • Six-rowed
  • Two-rowed

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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