Pyruvate protects giardia trophozoites from cysteine-ascorbate deprived medium induced cytotoxicity

Dibyendu Raj, Punam Chowdhury, Rituparna Sarkar, Yumiko Saito-Nakano, Keinosuke Okamoto, Shanta Dutta, Tomoyoshi Nozaki, Sandipan Ganguly

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Giardia lamblia, an anaerobic, amitochondriate protozoan parasite causes parasitic infection giardiasis in children and young adults. It produces pyruvate, a major metabolic product for its fermentative metabolism. The current study was undertaken to explore the effects of pyruvate as a physiological antioxidant during oxidative stress in Giardia by cysteine-ascorbate deprivation and further investigation upon the hypothesis that oxidative stress due to metabolism was the reason behind the cytotoxicity. We have estimated intracellular reactive oxygen species generation due to cysteine-ascorbate deprivation in Giardia. In the present study, we have examined the effects of extracellular addition of pyruvate, during oxidative stress generated from cysteine-ascorbate deprivation in culture media on DNA damage in Giardia. The intracellular pyruvate concentrations at several time points were measured in the trophozoites during stress. Trophozoites viability under cysteine-ascorbate deprived (CAD) medium in presence and absence of extracellular pyruvate has also been measured. The exogenous addition of a physiologically relevant concentration of pyruvate to trophozoites suspension was shown to attenuate the rate of ROS generation. We have demonstrated that Giardia protects itself from destructive consequences of ROS by maintaining the intracellular pyruvate concentration. Pyruvate recovers Giardia trophozoites from oxidative stress by decreasing the number of DNA breaks that might favor DNA repair.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalKorean Journal of Parasitology
Volume56
Issue number1
DOIs
Publication statusPublished - Feb 1 2018
Externally publishedYes

Fingerprint

Giardia
Trophozoites
Pyruvic Acid
Cysteine
Oxidative Stress
Giardiasis
Giardia lamblia
Parasitic Diseases
DNA Breaks
DNA Repair
DNA Damage
Culture Media
Young Adult
Reactive Oxygen Species
Suspensions
Parasites
Antioxidants

Keywords

  • Antioxidant
  • Cysteine-ascorbate
  • Cytotoxicity
  • Giardia lamblia
  • Oxidative stress
  • Pyruvate
  • Reactive oxygen species

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Pyruvate protects giardia trophozoites from cysteine-ascorbate deprived medium induced cytotoxicity. / Raj, Dibyendu; Chowdhury, Punam; Sarkar, Rituparna; Saito-Nakano, Yumiko; Okamoto, Keinosuke; Dutta, Shanta; Nozaki, Tomoyoshi; Ganguly, Sandipan.

In: Korean Journal of Parasitology, Vol. 56, No. 1, 01.02.2018, p. 1-9.

Research output: Contribution to journalArticle

Raj, D, Chowdhury, P, Sarkar, R, Saito-Nakano, Y, Okamoto, K, Dutta, S, Nozaki, T & Ganguly, S 2018, 'Pyruvate protects giardia trophozoites from cysteine-ascorbate deprived medium induced cytotoxicity', Korean Journal of Parasitology, vol. 56, no. 1, pp. 1-9. https://doi.org/10.3347/kjp.2018.56.1.1
Raj, Dibyendu ; Chowdhury, Punam ; Sarkar, Rituparna ; Saito-Nakano, Yumiko ; Okamoto, Keinosuke ; Dutta, Shanta ; Nozaki, Tomoyoshi ; Ganguly, Sandipan. / Pyruvate protects giardia trophozoites from cysteine-ascorbate deprived medium induced cytotoxicity. In: Korean Journal of Parasitology. 2018 ; Vol. 56, No. 1. pp. 1-9.
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