Pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the paired serum and cerebrospinal fluid of children

Tomoyuki Akiyama, Yumiko Hayashi, Yoshiyuki Hanaoka, Takashi Shibata, Mari Akiyama, Hiroki Tsuchiya, Tokito Yamaguchi, Katsuhiro Kobayashi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background We quantified pyridoxal 5′-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in paired serum and cerebrospinal fluid (CSF) samples from children and investigated the effect of age on the concentrations and CSF-to-serum ratios of these vitamers. Methods Serum and CSF samples prospectively collected from 49 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age on these vitamers, the PLP-to-PL ratio, CSF-to-serum PLP ratio, and CSF-to-serum PL ratio were evaluated using correlation analysis. Results The PLP, PL, and PA concentrations in the serum and CSF were higher at younger ages, except for CSF PA concentrations that were mostly below the limit of detection (< 1.2 nmol/l). The PLP-to-PL ratios in the serum and CSF correlated positively with age. The CSF-to-serum PLP ratio and CSF-to-serum PL ratio were independent of age. Conclusions Age-related changes in PLP, PL, and PA in serum and in CSF from pediatric patients and CSF-to-serum ratios of PLP and PL demonstrated in this study will provide valuable information for evaluating PLP supply to the central nervous system from the peripheral blood.

Original languageEnglish
Pages (from-to)118-122
Number of pages5
JournalClinica Chimica Acta
Volume472
DOIs
Publication statusPublished - Sep 1 2017

Fingerprint

Pyridoxic Acid
Pyridoxal
Cerebrospinal fluid
Pyridoxal Phosphate
Cerebrospinal Fluid
Serum
Pediatrics
High performance liquid chromatography
Neurology

Keywords

  • Blood-CSF barrier
  • Dephosphorylation
  • Fluorescence detection
  • Liquid chromatography
  • Phosphorylation
  • Transport
  • Vitamin B6

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the paired serum and cerebrospinal fluid of children. / Akiyama, Tomoyuki; Hayashi, Yumiko; Hanaoka, Yoshiyuki; Shibata, Takashi; Akiyama, Mari; Tsuchiya, Hiroki; Yamaguchi, Tokito; Kobayashi, Katsuhiro.

In: Clinica Chimica Acta, Vol. 472, 01.09.2017, p. 118-122.

Research output: Contribution to journalArticle

Akiyama, Tomoyuki ; Hayashi, Yumiko ; Hanaoka, Yoshiyuki ; Shibata, Takashi ; Akiyama, Mari ; Tsuchiya, Hiroki ; Yamaguchi, Tokito ; Kobayashi, Katsuhiro. / Pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the paired serum and cerebrospinal fluid of children. In: Clinica Chimica Acta. 2017 ; Vol. 472. pp. 118-122.
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AU - Shibata, Takashi

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