Pyrethroid-resistant diamondback moth expresses alternatively spliced sodium channel transcripts with and without T929I mutation

Shoji Sonoda, Chikako Igaki, Muhammad Ashfaq, Hisaaki Tsumuki

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This study revealed two distinct alternatively spliced exons, A1 and A2, encoding part of the domain IIS4-IIS5 of the para-sodium channel gene in the diamondback moth (DBM). Exons A1 and A2, respectively, revealed 79% and 91% identity at the nucleotide and amino acid levels. Both alternative exons included the T929I site, which has been associated with pyrethroid resistance in DBM. In the pyrethroid-resistant strain, susceptible (Thr) and resistant (Ile) amino acids were encoded at the T929I site in exons A1 and A2, respectively, but in the pyrethroid-susceptible strain, only Thr was encoded at the site in both exons. The transcripts containing exon A1 were expressed constitutively in all developmental stages. The transcripts containing exon A2 were also detected in all developmental stages, but the levels were significantly lower in the 3rd and 4th instar larvae. Tissue-specific data from the 4th instar larvae and adults showed that the expression of transcripts containing exon A2 was higher in heads than in bodies. These findings suggest that alternative splicing of the para-sodium channel gene might produce distinct channels with different sensitivities to pyrethroids, possibly in a tissue-specific manner.

Original languageEnglish
Pages (from-to)904-910
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume36
Issue number12
DOIs
Publication statusPublished - Dec 2006

Fingerprint

Pyrethrins
sodium channels
Moths
Sodium Channels
Plutella xylostella
alternative splicing
pyrethrins
exons
Exons
mutation
Mutation
varespladib methyl
Larva
instars
Genes
developmental stages
Tissue
Amino Acids
amino acids
larvae

Keywords

  • Alternative splicing
  • Diamondback moth
  • Insecticide
  • Knockdown resistance
  • Plutella xylostella
  • Pyrethroid
  • Sodium channel

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Pyrethroid-resistant diamondback moth expresses alternatively spliced sodium channel transcripts with and without T929I mutation. / Sonoda, Shoji; Igaki, Chikako; Ashfaq, Muhammad; Tsumuki, Hisaaki.

In: Insect Biochemistry and Molecular Biology, Vol. 36, No. 12, 12.2006, p. 904-910.

Research output: Contribution to journalArticle

Sonoda, Shoji ; Igaki, Chikako ; Ashfaq, Muhammad ; Tsumuki, Hisaaki. / Pyrethroid-resistant diamondback moth expresses alternatively spliced sodium channel transcripts with and without T929I mutation. In: Insect Biochemistry and Molecular Biology. 2006 ; Vol. 36, No. 12. pp. 904-910.
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