Putative start codon TTG for the regulatory protein VirG of the hairy-root-inducing plasmid pRiA4

Aoyama Takashi, Takashi Hirayama, Tamamoto Satoshi, Oka Atsuhiro

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The nucleotide sequence of the virG gene for a transcriptional activator on the agropine-type hairy-rootinducing plasmid pRiA4 was determined. The sequence contained one possible open reading frame. The gene product with a molecular size of 26.5 kDa was identified by an Escherichia coli coupled-transcription-translation system using cloned virG plasmids as templates. However, neither an ATG nor a GTG start codon which could give rise to such a protein was identified in the nucleotide sequence. Instead, TTG was found as a candidate for the start codon. This TTG was preceded, like most other TTG start codons, by both a Shine-Dalgarno (SD) sequence and a T signal which are respectively complementary to the 3'-end region of 16S rRNA and the Tψ/loop of initiator tRNA. Further evidence for the start at TTG was obtained by gene fusion experiments. When the E. coli lacZ gene, whose expression entirely depends on the transcription and translation from upstream regions, was connected in-phase with virG either directly upstream or downstream of the TTG sequence, only the latter fused gene expressed the β-galactosidase activity in Agrobacterium cells in response to a plant phenolic compound, acetosyringone. The TTG codon preceded by an SD sequence and a T signal is also conserved in the virG sequences from other three tumor-inducing plasmids previously reported.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalGene
Volume78
Issue number1
DOIs
Publication statusPublished - May 15 1989
Externally publishedYes

Fingerprint

Initiator Codon
Plasmids
Galactosidases
RNA, Transfer, Met
Escherichia coli
Genes
Agrobacterium
Proteins
Lac Operon
Gene Fusion
Codon
Open Reading Frames
Gene Expression
Neoplasms

Keywords

  • acetosyringone
  • agropine-type
  • plant phenolic compounds
  • plasmid evolution
  • positive regulator
  • Recombinant DNA
  • Ri and Ti plasmids
  • transcriptional activator
  • transformation
  • virulence gene

ASJC Scopus subject areas

  • Genetics

Cite this

Putative start codon TTG for the regulatory protein VirG of the hairy-root-inducing plasmid pRiA4. / Takashi, Aoyama; Hirayama, Takashi; Satoshi, Tamamoto; Atsuhiro, Oka.

In: Gene, Vol. 78, No. 1, 15.05.1989, p. 173-178.

Research output: Contribution to journalArticle

Takashi, Aoyama ; Hirayama, Takashi ; Satoshi, Tamamoto ; Atsuhiro, Oka. / Putative start codon TTG for the regulatory protein VirG of the hairy-root-inducing plasmid pRiA4. In: Gene. 1989 ; Vol. 78, No. 1. pp. 173-178.
@article{4cd19d96efe742d98b88557dc5b81933,
title = "Putative start codon TTG for the regulatory protein VirG of the hairy-root-inducing plasmid pRiA4",
abstract = "The nucleotide sequence of the virG gene for a transcriptional activator on the agropine-type hairy-rootinducing plasmid pRiA4 was determined. The sequence contained one possible open reading frame. The gene product with a molecular size of 26.5 kDa was identified by an Escherichia coli coupled-transcription-translation system using cloned virG plasmids as templates. However, neither an ATG nor a GTG start codon which could give rise to such a protein was identified in the nucleotide sequence. Instead, TTG was found as a candidate for the start codon. This TTG was preceded, like most other TTG start codons, by both a Shine-Dalgarno (SD) sequence and a T signal which are respectively complementary to the 3'-end region of 16S rRNA and the Tψ/loop of initiator tRNA. Further evidence for the start at TTG was obtained by gene fusion experiments. When the E. coli lacZ gene, whose expression entirely depends on the transcription and translation from upstream regions, was connected in-phase with virG either directly upstream or downstream of the TTG sequence, only the latter fused gene expressed the β-galactosidase activity in Agrobacterium cells in response to a plant phenolic compound, acetosyringone. The TTG codon preceded by an SD sequence and a T signal is also conserved in the virG sequences from other three tumor-inducing plasmids previously reported.",
keywords = "acetosyringone, agropine-type, plant phenolic compounds, plasmid evolution, positive regulator, Recombinant DNA, Ri and Ti plasmids, transcriptional activator, transformation, virulence gene",
author = "Aoyama Takashi and Takashi Hirayama and Tamamoto Satoshi and Oka Atsuhiro",
year = "1989",
month = "5",
day = "15",
doi = "10.1016/0378-1119(89)90325-9",
language = "English",
volume = "78",
pages = "173--178",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Putative start codon TTG for the regulatory protein VirG of the hairy-root-inducing plasmid pRiA4

AU - Takashi, Aoyama

AU - Hirayama, Takashi

AU - Satoshi, Tamamoto

AU - Atsuhiro, Oka

PY - 1989/5/15

Y1 - 1989/5/15

N2 - The nucleotide sequence of the virG gene for a transcriptional activator on the agropine-type hairy-rootinducing plasmid pRiA4 was determined. The sequence contained one possible open reading frame. The gene product with a molecular size of 26.5 kDa was identified by an Escherichia coli coupled-transcription-translation system using cloned virG plasmids as templates. However, neither an ATG nor a GTG start codon which could give rise to such a protein was identified in the nucleotide sequence. Instead, TTG was found as a candidate for the start codon. This TTG was preceded, like most other TTG start codons, by both a Shine-Dalgarno (SD) sequence and a T signal which are respectively complementary to the 3'-end region of 16S rRNA and the Tψ/loop of initiator tRNA. Further evidence for the start at TTG was obtained by gene fusion experiments. When the E. coli lacZ gene, whose expression entirely depends on the transcription and translation from upstream regions, was connected in-phase with virG either directly upstream or downstream of the TTG sequence, only the latter fused gene expressed the β-galactosidase activity in Agrobacterium cells in response to a plant phenolic compound, acetosyringone. The TTG codon preceded by an SD sequence and a T signal is also conserved in the virG sequences from other three tumor-inducing plasmids previously reported.

AB - The nucleotide sequence of the virG gene for a transcriptional activator on the agropine-type hairy-rootinducing plasmid pRiA4 was determined. The sequence contained one possible open reading frame. The gene product with a molecular size of 26.5 kDa was identified by an Escherichia coli coupled-transcription-translation system using cloned virG plasmids as templates. However, neither an ATG nor a GTG start codon which could give rise to such a protein was identified in the nucleotide sequence. Instead, TTG was found as a candidate for the start codon. This TTG was preceded, like most other TTG start codons, by both a Shine-Dalgarno (SD) sequence and a T signal which are respectively complementary to the 3'-end region of 16S rRNA and the Tψ/loop of initiator tRNA. Further evidence for the start at TTG was obtained by gene fusion experiments. When the E. coli lacZ gene, whose expression entirely depends on the transcription and translation from upstream regions, was connected in-phase with virG either directly upstream or downstream of the TTG sequence, only the latter fused gene expressed the β-galactosidase activity in Agrobacterium cells in response to a plant phenolic compound, acetosyringone. The TTG codon preceded by an SD sequence and a T signal is also conserved in the virG sequences from other three tumor-inducing plasmids previously reported.

KW - acetosyringone

KW - agropine-type

KW - plant phenolic compounds

KW - plasmid evolution

KW - positive regulator

KW - Recombinant DNA

KW - Ri and Ti plasmids

KW - transcriptional activator

KW - transformation

KW - virulence gene

UR - http://www.scopus.com/inward/record.url?scp=0024970322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024970322&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(89)90325-9

DO - 10.1016/0378-1119(89)90325-9

M3 - Article

C2 - 2670679

AN - SCOPUS:0024970322

VL - 78

SP - 173

EP - 178

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -