Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor

Gregory A. Mignery, Thomas C. Südhof, Kohji Takei, Pietro De Camilli

Research output: Contribution to journalArticle

380 Citations (Scopus)

Abstract

INOSITOL 1,4,5-trisphosphate (Ins(l,4,5)P3) serves as an intracellular second messenger for several neurotransmitters, hormones and growth factors by initiating calcium release from intracellular stores1,2. A cerebellar Ins(l,4,5)P3 receptor has been characterized biochemically3,4 and shown by immunocytochemistry to be present in intracellular membranes in Purkinje cells5. We show that a previously described Purkinje-cell messenger RNA6 encodes a protein of relative molecular mass 260,000 (260 K) with the same properties as the cerebellar Ins(l,4,5)P3 receptor. Its sequence is partially homologous to the skeletal muscle ryanodine receptor7. By immunocytochemistry and electron microscopy the protein is shown to be present in all parts of the endoplasmic reticulum, including those that extend into axon terminals and dendritic spines. Our results indicate that gated calcium release from intracellular stores in muscle and Purkinje cells uses similar calcium-channel proteins localized in analogous intracellular compartments. This implies that the intracellular calcium stores in the endoplasmic reticulum of neurons extend into presynaptic terminals and dendritic spines where they may play a direct role in regulating the efficacy of neurotransmission.

Original languageEnglish
Pages (from-to)192-195
Number of pages4
JournalNature
Volume342
Issue number6246
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Ryanodine Receptor Calcium Release Channel
Dendritic Spines
Purkinje Cells
Presynaptic Terminals
Calcium
Endoplasmic Reticulum
Immunohistochemistry
Ryanodine
Intracellular Membranes
Proteins
Second Messenger Systems
Calcium Channels
Synaptic Transmission
Muscle Cells
Neurotransmitter Agents
Intercellular Signaling Peptides and Proteins
Electron Microscopy
Skeletal Muscle
Hormones

ASJC Scopus subject areas

  • General

Cite this

Mignery, G. A., Südhof, T. C., Takei, K., & De Camilli, P. (1989). Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor. Nature, 342(6246), 192-195.

Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor. / Mignery, Gregory A.; Südhof, Thomas C.; Takei, Kohji; De Camilli, Pietro.

In: Nature, Vol. 342, No. 6246, 1989, p. 192-195.

Research output: Contribution to journalArticle

Mignery, GA, Südhof, TC, Takei, K & De Camilli, P 1989, 'Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor', Nature, vol. 342, no. 6246, pp. 192-195.
Mignery GA, Südhof TC, Takei K, De Camilli P. Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor. Nature. 1989;342(6246):192-195.
Mignery, Gregory A. ; Südhof, Thomas C. ; Takei, Kohji ; De Camilli, Pietro. / Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor. In: Nature. 1989 ; Vol. 342, No. 6246. pp. 192-195.
@article{a2392d97091a4157ab76ed8fd9293ca4,
title = "Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor",
abstract = "INOSITOL 1,4,5-trisphosphate (Ins(l,4,5)P3) serves as an intracellular second messenger for several neurotransmitters, hormones and growth factors by initiating calcium release from intracellular stores1,2. A cerebellar Ins(l,4,5)P3 receptor has been characterized biochemically3,4 and shown by immunocytochemistry to be present in intracellular membranes in Purkinje cells5. We show that a previously described Purkinje-cell messenger RNA6 encodes a protein of relative molecular mass 260,000 (260 K) with the same properties as the cerebellar Ins(l,4,5)P3 receptor. Its sequence is partially homologous to the skeletal muscle ryanodine receptor7. By immunocytochemistry and electron microscopy the protein is shown to be present in all parts of the endoplasmic reticulum, including those that extend into axon terminals and dendritic spines. Our results indicate that gated calcium release from intracellular stores in muscle and Purkinje cells uses similar calcium-channel proteins localized in analogous intracellular compartments. This implies that the intracellular calcium stores in the endoplasmic reticulum of neurons extend into presynaptic terminals and dendritic spines where they may play a direct role in regulating the efficacy of neurotransmission.",
author = "Mignery, {Gregory A.} and S{\"u}dhof, {Thomas C.} and Kohji Takei and {De Camilli}, Pietro",
year = "1989",
language = "English",
volume = "342",
pages = "192--195",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6246",

}

TY - JOUR

T1 - Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor

AU - Mignery, Gregory A.

AU - Südhof, Thomas C.

AU - Takei, Kohji

AU - De Camilli, Pietro

PY - 1989

Y1 - 1989

N2 - INOSITOL 1,4,5-trisphosphate (Ins(l,4,5)P3) serves as an intracellular second messenger for several neurotransmitters, hormones and growth factors by initiating calcium release from intracellular stores1,2. A cerebellar Ins(l,4,5)P3 receptor has been characterized biochemically3,4 and shown by immunocytochemistry to be present in intracellular membranes in Purkinje cells5. We show that a previously described Purkinje-cell messenger RNA6 encodes a protein of relative molecular mass 260,000 (260 K) with the same properties as the cerebellar Ins(l,4,5)P3 receptor. Its sequence is partially homologous to the skeletal muscle ryanodine receptor7. By immunocytochemistry and electron microscopy the protein is shown to be present in all parts of the endoplasmic reticulum, including those that extend into axon terminals and dendritic spines. Our results indicate that gated calcium release from intracellular stores in muscle and Purkinje cells uses similar calcium-channel proteins localized in analogous intracellular compartments. This implies that the intracellular calcium stores in the endoplasmic reticulum of neurons extend into presynaptic terminals and dendritic spines where they may play a direct role in regulating the efficacy of neurotransmission.

AB - INOSITOL 1,4,5-trisphosphate (Ins(l,4,5)P3) serves as an intracellular second messenger for several neurotransmitters, hormones and growth factors by initiating calcium release from intracellular stores1,2. A cerebellar Ins(l,4,5)P3 receptor has been characterized biochemically3,4 and shown by immunocytochemistry to be present in intracellular membranes in Purkinje cells5. We show that a previously described Purkinje-cell messenger RNA6 encodes a protein of relative molecular mass 260,000 (260 K) with the same properties as the cerebellar Ins(l,4,5)P3 receptor. Its sequence is partially homologous to the skeletal muscle ryanodine receptor7. By immunocytochemistry and electron microscopy the protein is shown to be present in all parts of the endoplasmic reticulum, including those that extend into axon terminals and dendritic spines. Our results indicate that gated calcium release from intracellular stores in muscle and Purkinje cells uses similar calcium-channel proteins localized in analogous intracellular compartments. This implies that the intracellular calcium stores in the endoplasmic reticulum of neurons extend into presynaptic terminals and dendritic spines where they may play a direct role in regulating the efficacy of neurotransmission.

UR - http://www.scopus.com/inward/record.url?scp=0024449839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024449839&partnerID=8YFLogxK

M3 - Article

VL - 342

SP - 192

EP - 195

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6246

ER -