Purkinje cells in the adult cat cerebellar cortex possess a perineuronal net of proteoglycans

M. Mabuchi, S. Murakami, Takehito Taguchi, Aiji Ohtsuka, T. Murakami

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The Purkinje cells in the adult cat cerebellar cortex were found to possess perineuronal proteoglycans which could be stained with our tine cationic iron colloid and Fujita's highly concentrated aldehyde fuchsin, and digested by chondroitinase ABC/keratanase/heparitinase and hyaluronidase. The Purkinje cells are surrounded by some collagenous elements which are stained with Gömöri's ammoniacal silver and digested by collagenase. The Purkinje cells also express nerve cell surface glycoproteins which are labeled with lectin Vicia villosa agglutinin and digested by a double treatment with collagenase and endo-alpha-N-acetylgalactosaminidase. Sole digestion by endo-alpha-N-acetylgalactosaminidase never erased the lectin labeling of the nerve cell surface glycoproteins. These findings suggest that the collagenous elements mediate the linkage of the perineuronal proteoglycans to the nerve cell surface glycoproteins. It is presumed that in mice and rats, the perineuronal nets of proteoglycans and nerve cell surface glycoproteins of the Purkinje cells are so thin or coarse that they can not be sufficiently visualized under the light microscope.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalArchives of Histology and Cytology
Volume64
Issue number2
Publication statusPublished - 2001

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Cerebellar Cortex
Purkinje Cells
Membrane Glycoproteins
Proteoglycans
alpha-N-Acetylgalactosaminidase
Cats
Neurons
keratan-sulfate endo-1,4-beta-galactosidase
Collagenases
heparitinsulfate lyase
Lectins
Chondroitin ABC Lyase
Rosaniline Dyes
Hyaluronoglucosaminidase
Colloids
Aldehydes
Digestion
Iron
Light

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Purkinje cells in the adult cat cerebellar cortex possess a perineuronal net of proteoglycans. / Mabuchi, M.; Murakami, S.; Taguchi, Takehito; Ohtsuka, Aiji; Murakami, T.

In: Archives of Histology and Cytology, Vol. 64, No. 2, 2001, p. 203-209.

Research output: Contribution to journalArticle

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