Purified culture systems for bovine oviductal stromal cells

Yuki Yamamoto, Yoshihiko Kobayashi, Kiyoshi Okuda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Isolated stromal cells from the ampullary and isthmic parts of bovine oviductal tissues were cultured in monolayer and spheroid (cell aggregate) systems. Prostaglandin F2α (PGF) plays a crucial role in oviductal contraction and is produced by oviductal epithelial cells in cattle. Since stromal cells of many organs produce PGF, PGF production by bovine oviductal stromal cells was investigated. After PGF synthesis was confirmed, the utility of isolation and culture methods for oviductal stromal cells was evaluated by PGF production in the present study. The homogeneity of the cells was > 99%. PGF production of the cells was increased by tumor necrosis factor-α. The stromal cells aggregated and formed a spheroid by the treatments with several reagents. PGF production was higher in the spheroid culture than in the monolayer culture. The isolation and culture methods described here will facilitate studies of the physiological function of bovine oviductal stromal cells.

Original languageEnglish
Pages (from-to)73-77
Number of pages5
JournalJournal of Reproduction and Development
Volume60
Issue number1
DOIs
Publication statusPublished - 2014

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stromal cells
prostaglandins
cattle
tumor necrosis factors
cell aggregates
epithelial cells
cells
synthesis
methodology

Keywords

  • Cell culture
  • Cell-to-cell interaction
  • Cow
  • Oviductal stromal cell
  • Spheroid

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Purified culture systems for bovine oviductal stromal cells. / Yamamoto, Yuki; Kobayashi, Yoshihiko; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 60, No. 1, 2014, p. 73-77.

Research output: Contribution to journalArticle

Yamamoto, Yuki ; Kobayashi, Yoshihiko ; Okuda, Kiyoshi. / Purified culture systems for bovine oviductal stromal cells. In: Journal of Reproduction and Development. 2014 ; Vol. 60, No. 1. pp. 73-77.
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