Purification of sinus gland peptides having vitellogenesis-inhibiting activity from the whiteleg shrimp Litopenaeus vannamei

Naoaki Tsutsui, Tsuyoshi Ohira, Ichiro Kawazoe, Akiyoshi Takahashi, Marcy N. Wilder

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. To characterize multiple VIH molecules in the whiteleg shrimp Litopenaeus vannamei, seven CHH-family peptides designated as Liv-SGP-A, -B, -C, -D, -E, -F, and -G were purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The dose-response effects of these peptides on vitellogenin mRNA levels were examined using in vitro incubation of ovarian fragments of the kuruma prawn Marsupenaeus japonicus. Liv-SGP-D showed no significant inhibitory activities, while the other six peptides significantly reduced vitellogenin mRNA levels, however, with differing efficacies, in the order of Liv-SGP-C, -F, -G > -A, -B > -E. Liv-SGP-G was the most abundant CHH-family peptide in the sinus gland and showed strong vitellogenesis-inhibiting activity. As a result of detailed structural analysis, its complete primary structure was determined; it consisted of 72 amino acid residues and possesses an amidated C-terminus.

Original languageEnglish
Pages (from-to)360-369
Number of pages10
JournalMarine Biotechnology
Volume9
Issue number3
DOIs
Publication statusPublished - May 1 2007

Keywords

  • Crustacean hyperglycemic hormone-family peptides
  • Litopenaeus vannamei
  • Marsupenaeus japonicus
  • Sinus glands
  • Vitellogenesis-inhibiting hormone
  • Vitellogenin

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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