Purification of sinus gland peptides having vitellogenesis-inhibiting activity from the whiteleg shrimp Litopenaeus vannamei

Naoaki Tsutsui, Tsuyoshi Ohira, Ichiro Kawazoe, Akiyoshi Takahashi, Marcy N. Wilder

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. To characterize multiple VIH molecules in the whiteleg shrimp Litopenaeus vannamei, seven CHH-family peptides designated as Liv-SGP-A, -B, -C, -D, -E, -F, and -G were purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The dose-response effects of these peptides on vitellogenin mRNA levels were examined using in vitro incubation of ovarian fragments of the kuruma prawn Marsupenaeus japonicus. Liv-SGP-D showed no significant inhibitory activities, while the other six peptides significantly reduced vitellogenin mRNA levels, however, with differing efficacies, in the order of Liv-SGP-C, -F, -G > -A, -B > -E. Liv-SGP-G was the most abundant CHH-family peptide in the sinus gland and showed strong vitellogenesis-inhibiting activity. As a result of detailed structural analysis, its complete primary structure was determined; it consisted of 72 amino acid residues and possesses an amidated C-terminus.

Original languageEnglish
Pages (from-to)360-369
Number of pages10
JournalMarine Biotechnology
Volume9
Issue number3
DOIs
Publication statusPublished - May 2007
Externally publishedYes

Fingerprint

Vitellogenesis
vitellogenesis
Litopenaeus vannamei
sinuses
peptide
hormone
Purification
purification
hormones
peptides
Vitellogenins
Peptides
Crustacea
crustacean
Marsupenaeus japonicus
vitellogenin
Amino Acids
Messenger RNA
amino acid
Protein Sequence Analysis

Keywords

  • Crustacean hyperglycemic hormone-family peptides
  • Litopenaeus vannamei
  • Marsupenaeus japonicus
  • Sinus glands
  • Vitellogenesis-inhibiting hormone
  • Vitellogenin

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology
  • Biochemistry

Cite this

Purification of sinus gland peptides having vitellogenesis-inhibiting activity from the whiteleg shrimp Litopenaeus vannamei. / Tsutsui, Naoaki; Ohira, Tsuyoshi; Kawazoe, Ichiro; Takahashi, Akiyoshi; Wilder, Marcy N.

In: Marine Biotechnology, Vol. 9, No. 3, 05.2007, p. 360-369.

Research output: Contribution to journalArticle

Tsutsui, Naoaki ; Ohira, Tsuyoshi ; Kawazoe, Ichiro ; Takahashi, Akiyoshi ; Wilder, Marcy N. / Purification of sinus gland peptides having vitellogenesis-inhibiting activity from the whiteleg shrimp Litopenaeus vannamei. In: Marine Biotechnology. 2007 ; Vol. 9, No. 3. pp. 360-369.
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