Purification of a serine protease of Vibrio parahaemolyticus and its characterization

Masami Ishihara, Ayako Kawanishi, Hirofumi Watanabe, Ken Ichi Tomochika, Shin-ichi Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A 50 kDa protease designated as VPP1 was purified from the culture supernatant of a clinical strain of Vibrio parahaemolyticus by ammonium sulfate fractionation, Sephacryl S-200 HR gel filtration and Fractogel EMD TMAE 650 ion-exchange chromatography. VPP1 was inhibited by EDTA, EGTA and serine protease inhibitors, suggesting that it is a calcium-dependent serine protease. N-terminal amino acid sequence of VPP1 was quite similar to that of V. metschnikovii protease and antibody against VPP1 inhibited the activity of V. metschnikovii protease, suggesting the similarity of the two proteases. It was demonstrated that VPP1 or its related protease widely distribute in not only V. parahaemolyticus but also V. alginolyticus.

Original languageEnglish
Pages (from-to)299-303
Number of pages5
JournalMicrobiology and Immunology
Volume46
Issue number4
Publication statusPublished - 2002

Fingerprint

Vibrio parahaemolyticus
Serine Proteases
Peptide Hydrolases
Serine Proteinase Inhibitors
Egtazic Acid
Ion Exchange Chromatography
Ammonium Sulfate
Edetic Acid
Gel Chromatography
Amino Acid Sequence
Calcium
Antibodies
protease V

Keywords

  • Serine protease
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Ishihara, M., Kawanishi, A., Watanabe, H., Tomochika, K. I., Miyoshi, S., & Shinoda, S. (2002). Purification of a serine protease of Vibrio parahaemolyticus and its characterization. Microbiology and Immunology, 46(4), 299-303.

Purification of a serine protease of Vibrio parahaemolyticus and its characterization. / Ishihara, Masami; Kawanishi, Ayako; Watanabe, Hirofumi; Tomochika, Ken Ichi; Miyoshi, Shin-ichi; Shinoda, Sumio.

In: Microbiology and Immunology, Vol. 46, No. 4, 2002, p. 299-303.

Research output: Contribution to journalArticle

Ishihara, M, Kawanishi, A, Watanabe, H, Tomochika, KI, Miyoshi, S & Shinoda, S 2002, 'Purification of a serine protease of Vibrio parahaemolyticus and its characterization', Microbiology and Immunology, vol. 46, no. 4, pp. 299-303.
Ishihara M, Kawanishi A, Watanabe H, Tomochika KI, Miyoshi S, Shinoda S. Purification of a serine protease of Vibrio parahaemolyticus and its characterization. Microbiology and Immunology. 2002;46(4):299-303.
Ishihara, Masami ; Kawanishi, Ayako ; Watanabe, Hirofumi ; Tomochika, Ken Ichi ; Miyoshi, Shin-ichi ; Shinoda, Sumio. / Purification of a serine protease of Vibrio parahaemolyticus and its characterization. In: Microbiology and Immunology. 2002 ; Vol. 46, No. 4. pp. 299-303.
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