Purification of a novel serpin-like protein from bovine brain

Masahiro Nishibori, Takashi Chikai, Masahiro Kawabata, Jun Ohta, Toshihiko Ubuka, Kiyomi Saeki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We purified a novel serine proteinase inhibitor (serpin)-like protein from the bovine brain and named it B-43 from its molecular mass, 43 kDa. A cleaved peptide from B-43 was copurified with the native B-43. Partial amino acid sequencing of the purified B-43 showed that this protein was homologous to glia-derived nexin/protease nexin-1 (GDN/PN-1), plasminogen activator inhibitor 2, leukocyte elastase inhibitor (LEI) and placental thrombin inhibitor (PTI) among the serpins. Although B-43 had a similar amino acid composition to these serpins, the biochemical features of B-43 were different from them. B-43 did not form sodium dodecyl sulfate (SDS)-resistant serpin-proteinase complexes with thrombin, urokinase, pancreatic elastase and plasmin, suggesting that these proteinases were not the targets of B-43. In contrast to GDN/PN-1, B-43 did not have an affinity for heparin. B-43, having different biochemical properties from GDN/PN-1, appears to be an additional serpin expressed in the brain.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalNeuroscience Research
Volume24
Issue number1
DOIs
Publication statusPublished - 1995

Fingerprint

Serine Proteinase Inhibitors
Brain
Protease Nexins
Proteins
Neuroglia
Serpins
Peptide Hydrolases
Plasminogen Activator Inhibitor 2
B 43
Leukocyte Elastase
Pancreatic Elastase
Fibrinolysin
Protein Sequence Analysis
Urokinase-Type Plasminogen Activator
Thrombin
Sodium Dodecyl Sulfate
Heparin
Amino Acids

Keywords

  • Bovine brain
  • Glia-derived nexin/protease nexin-1
  • Serine proteinase
  • Serine proteinase inhibitor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Purification of a novel serpin-like protein from bovine brain. / Nishibori, Masahiro; Chikai, Takashi; Kawabata, Masahiro; Ohta, Jun; Ubuka, Toshihiko; Saeki, Kiyomi.

In: Neuroscience Research, Vol. 24, No. 1, 1995, p. 47-52.

Research output: Contribution to journalArticle

Nishibori, Masahiro ; Chikai, Takashi ; Kawabata, Masahiro ; Ohta, Jun ; Ubuka, Toshihiko ; Saeki, Kiyomi. / Purification of a novel serpin-like protein from bovine brain. In: Neuroscience Research. 1995 ; Vol. 24, No. 1. pp. 47-52.
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