Purification of a lethal toxin produced by Shigella dysenteriae

Keinosuke Okamoto, Yoshifumi Takeda, Toshio Miwatani

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Abstract

A lethal toxin was purified from the culture supernatant of Shigella dysenteriae 1. The purification procedure utilized ammonium sulfate fractionation, column chromatography on DEAE-cellulose, CM-cellulose, hydroxylapatite and gel filtration on Sephadex G-200. About a 4760-fold purification was achieved, with a yield of 2.7%. The purified lethal toxin appeared homogeneous on polyacrylamide gel disc electrophoresis. The ld50 of the purified lethal toxin in mice following i.p. injection was about 7.5 μg/kg. Injection i.p. of 2 ld50 of the purified lethal toxin into mice caused paralysis of the limbs followed by death after 2-4 days.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalToxicon
Volume20
Issue number2
DOIs
Publication statusPublished - 1982
Externally publishedYes

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ASJC Scopus subject areas

  • Toxicology

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