TY - JOUR
T1 - Purification of a lethal toxin produced by Shigella dysenteriae
AU - Okamoto, Keinosuke
AU - Takeda, Yoshifumi
AU - Miwatani, Toshio
N1 - Funding Information:
Acknowledgement-This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Stieence and Ctitlture of Japan .
PY - 1982
Y1 - 1982
N2 - A lethal toxin was purified from the culture supernatant of Shigella dysenteriae 1. The purification procedure utilized ammonium sulfate fractionation, column chromatography on DEAE-cellulose, CM-cellulose, hydroxylapatite and gel filtration on Sephadex G-200. About a 4760-fold purification was achieved, with a yield of 2.7%. The purified lethal toxin appeared homogeneous on polyacrylamide gel disc electrophoresis. The ld50 of the purified lethal toxin in mice following i.p. injection was about 7.5 μg/kg. Injection i.p. of 2 ld50 of the purified lethal toxin into mice caused paralysis of the limbs followed by death after 2-4 days.
AB - A lethal toxin was purified from the culture supernatant of Shigella dysenteriae 1. The purification procedure utilized ammonium sulfate fractionation, column chromatography on DEAE-cellulose, CM-cellulose, hydroxylapatite and gel filtration on Sephadex G-200. About a 4760-fold purification was achieved, with a yield of 2.7%. The purified lethal toxin appeared homogeneous on polyacrylamide gel disc electrophoresis. The ld50 of the purified lethal toxin in mice following i.p. injection was about 7.5 μg/kg. Injection i.p. of 2 ld50 of the purified lethal toxin into mice caused paralysis of the limbs followed by death after 2-4 days.
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U2 - 10.1016/0041-0101(82)90008-3
DO - 10.1016/0041-0101(82)90008-3
M3 - Article
C2 - 7043787
AN - SCOPUS:0020073221
SN - 0041-0101
VL - 20
SP - 451
EP - 456
JO - Toxicon
JF - Toxicon
IS - 2
ER -