Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defese cytokines

Teizo Yoshimura, Kouji Matsushima, Shuji Tanaka, Elizabeth A. Robinson, Ettoe Appella, Joost J. Oppenheim, Adward J. Leonard

Research output: Contribution to journalArticle

Abstract

Stimulated human monocytes release several proteins thought to play a role in inflammation, including interleukin 1, tumor necrosis factor, and plasminogen activator. We have purified another proinflammatory protein that is chemotactic for human neutrophils from conditioned medium of lipopolysaccharide-stimulated monocytes. After a series of steps that included anion-exchange chromatography, gel filtration, and HPLC on catio-exchange and reverse-phase columns, an apparently pure protein was obtained that migrated as a single 7-kDa bacd on NaDodSO4/polyacrylamide gels under reducing or nonreducing conditions. The amino acid composition of this monocyte-derived neutrophil chemotactic factor was different from that of interleukin 1 and tumor necrosis factor. N-terminal amino acid sequence of th first 42 residues was determined. This portion of the molecule has up to 56% sequence similarity with several proteins that may be involved in host responses to infection or tissue injury. It is identical to a portion of a sequence deduced from an mRNA induced by staphylococcal enterotoxin treatment of human leukocytes. At the optiomal concentration of 10nM, 50% of nutorophils added to chemotaxi assay wells migreated toward the pure attractant. Potency and efficacy ae comparable to that of fMet-Leu-Phe, which is often used as a reference. In contrast to many attractants, the protein was not chemotactic for human monocytes.

Original languageEnglish
Pages (from-to)5-9
Number of pages5
JournalJournal of Immunology
Volume202
Issue number1
Publication statusPublished - Jan 1 2019
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Yoshimura, T., Matsushima, K., Tanaka, S., Robinson, E. A., Appella, E., Oppenheim, J. J., & Leonard, A. J. (2019). Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defese cytokines. Journal of Immunology, 202(1), 5-9.