Purification of a cytochrome P450 isozyme belonging to a subfamily of P450 IIB from liver microsomes of guinea pigs

Shizuo Narimatus, Yuko Akutsu, Tamihide Matsunaga, Kazuhito Watanabe, Ikuo Yamamoto, Hidetoshi Yoshimura

Research output: Contribution to journalArticle

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Abstract

An isozyme of cytochrome P450 was purified from liver microsomes of guinea pigs by HPLC with anion-exchange and hydroxylaptite columns. The isozyme showed a single band of 52 kdalton on sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Oxidation activities of the final preparation towards p-nitroanisole, aniline and d-benzphetamine were 1.3 to 15.4-fold comparing with those of microsomal fraction. This isozyme was also concerned with oxidation of Δ9-tetrahydrocannabinol (THC) to 8α-hydroxy- (8α-OH-), 2′-OH- and 3′-OH-Δ9-THCs. The N-terminal region of the isozyme was considerably hydrophobic, and 13 of the first 20 amino acid residues was leucine. Since this first 20 amino acid sequence is 80% homologous with that of cytochrome P450 LM2 purified from rabbit liver microsomes, this isozyme can be categorized to a subfamily of P450 IIB.

Original languageEnglish
Pages (from-to)607-613
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number2
DOIs
Publication statusPublished - Oct 30 1990
Externally publishedYes

Fingerprint

Liver Microsomes
Liver
Cytochrome P-450 Enzyme System
Isoenzymes
Purification
Guinea Pigs
Dronabinol
Benzphetamine
Amino Acids
Oxidation
Electrophoresis
Leucine
Anions
Amino Acid Sequence
Gels
Sodium
High Pressure Liquid Chromatography
Rabbits
hydroxide ion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Purification of a cytochrome P450 isozyme belonging to a subfamily of P450 IIB from liver microsomes of guinea pigs. / Narimatus, Shizuo; Akutsu, Yuko; Matsunaga, Tamihide; Watanabe, Kazuhito; Yamamoto, Ikuo; Yoshimura, Hidetoshi.

In: Biochemical and Biophysical Research Communications, Vol. 172, No. 2, 30.10.1990, p. 607-613.

Research output: Contribution to journalArticle

Narimatus, Shizuo ; Akutsu, Yuko ; Matsunaga, Tamihide ; Watanabe, Kazuhito ; Yamamoto, Ikuo ; Yoshimura, Hidetoshi. / Purification of a cytochrome P450 isozyme belonging to a subfamily of P450 IIB from liver microsomes of guinea pigs. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 172, No. 2. pp. 607-613.
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