Purification, identification, and cDNA cloning of Cha o 2, the second major allergen of Japanese cypress pollen

Takeshi Mori, Minehiko Yokoyama, Naoki Komiyama, Mitsuhiro Okano, Kohsuke Kino

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The second major allergen of Chamaecyparis obtusa (Japanese cypress) pollen, Cha o 2, has been purified and its cDNA cloned. Of patients with pollinosis caused by C. obtusa, 82.5% produce IgE antibodies which react with purified Cha o 2. The purified protein has a molecular mass of 46 kDa and its 12 N-terminal amino acid sequence displays a high homology with that of Cry j 2, the second major allergen of Cryptomeria japonica pollen. cDNA clones coding for Cha o 2 have been isolated using Cry j 2 cDNA as a probe. Cha o 2 cDNA clones were sequenced and found to code a putative 50-residue signal sequence and a 464-residue mature protein with a molecular weight of 50 kDa. Two possible N-linked glycosylation sites were found in the sequence. The deduced amino acid sequence of Cha o 2 shows 74.3% identity with that of Cry j 2. In its primary structure, Cha o 2 shows significant identity with those of the polygalacturonases of avocado, tomato, and maize as well as Cry j 2.

Original languageEnglish
Pages (from-to)166-171
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume263
Issue number1
DOIs
Publication statusPublished - Sep 16 1999

Fingerprint

Cupressus
Cloning
Pollen
Allergens
Purification
Organism Cloning
Chamaecyparis
Complementary DNA
Amino Acid Sequence
Clone Cells
Cryptomeria
Persea
Glycosylation
Polygalacturonase
Amino Acids
Seasonal Allergic Rhinitis
Molecular mass
Lycopersicon esculentum
Protein Sorting Signals
Immunoglobulin E

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Purification, identification, and cDNA cloning of Cha o 2, the second major allergen of Japanese cypress pollen. / Mori, Takeshi; Yokoyama, Minehiko; Komiyama, Naoki; Okano, Mitsuhiro; Kino, Kohsuke.

In: Biochemical and Biophysical Research Communications, Vol. 263, No. 1, 16.09.1999, p. 166-171.

Research output: Contribution to journalArticle

Mori, Takeshi ; Yokoyama, Minehiko ; Komiyama, Naoki ; Okano, Mitsuhiro ; Kino, Kohsuke. / Purification, identification, and cDNA cloning of Cha o 2, the second major allergen of Japanese cypress pollen. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 263, No. 1. pp. 166-171.
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