Purification and some properties of sulfur: ferric ion oxidoreductase from Thiobacillus ferrooxidans.

T. Sugio, W. Mizunashi, Kenji Inagaki, T. Tano

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Abstract

A sulfur:ferric ion oxidoreductase that utilizes ferric ion (Fe3+) as an electron acceptor of elemental sulfur was purified from iron-grown Thiobacillus ferrooxidans to an electrophoretically homogeneous state. Under anaerobic conditions in the presence of Fe3+, the enzyme reduced 4 mol of Fe3+ with 1 mol of elemental sulfur to give 4 mol of Fe2+ and 1 mol of sulfite, indicating that it corresponds to a ferric ion-reducing system (T. Sugio, C. Domatsu, O. Munakata, T. Tano, and K. Imai, Appl. Environ. Microbiol. 49:1401-1406, 1985). Under aerobic conditions, sulfite, but not Fe2+, was produced during the oxidation of elemental sulfur by this enzyme because the Fe2+ produced was rapidly reoxidized chemically by molecular oxygen. The possibility that Fe3+ serves as an electron acceptor under aerobic conditions was ascertained by adding o-phenanthroline, which chelates Fe2+, to the reaction mixture. Sulfur:ferric ion oxidoreductase had an apparent molecular weight of 46,000, and it is composed of two identical subunits (Mr = 23,000) as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sulfur oxidation by this enzyme was absolutely dependent on the presence of reduced glutathione. The enzyme had an isoelectric point and a pH optimum at pH 4.6 and 6.5, respectively. Almost all the activity of sulfur:ferric ion oxidoreductase was observed in the osmotic shock fluid of the cells, suggesting that it was localized in the periplasmic space of the cells.

Original languageEnglish
Pages (from-to)4916-4922
Number of pages7
JournalJournal of Bacteriology
Volume169
Issue number11
Publication statusPublished - Nov 1987

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Thiobacillus
Sulfur
Sulfites
Enzymes
Electrons
Ions
Periplasm
Osmotic Pressure
Isoelectric Point
Sodium Dodecyl Sulfate
Glutathione
Polyacrylamide Gel Electrophoresis
Iron
Molecular Weight
Oxygen
sulfur-ferric ion oxidoreductase

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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Purification and some properties of sulfur : ferric ion oxidoreductase from Thiobacillus ferrooxidans. / Sugio, T.; Mizunashi, W.; Inagaki, Kenji; Tano, T.

In: Journal of Bacteriology, Vol. 169, No. 11, 11.1987, p. 4916-4922.

Research output: Contribution to journalArticle

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