Purification and some properties of inducible lysine decarboxylase from Vibrio parahaemolyticus

S. Yamamoto, T. Imamura, K. Kusaba, S. Shinoda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Inducible lysine decarboxylase from Vibrio parahaemolyticus AQ 3627 was purified to apparent homogeneity and characterized. The enzyme displayed a molecular weight of 531000 by gel filtration and 79000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme required pyridoxal phosphate as a cofactor, and the pH optimum was 5.5. The K(m) value for L-lysine was 3.2 mM, and the enzyme was inhibited by 6-aminocaproic acid and α-fluoromethyl analogs of cadaverine. δ-Hydroxylysine and S-aminoethyl-L-cysteine was active as substrates to a lesser extent than L-lysine. The amino-terminal amino acid sequence was determined to be Met-Asn-Ile-Phe-Ala-Ile-Leu. These properties were compared with those of other bacterial lysine decarboxylases.

Original languageEnglish
Pages (from-to)3067-3070
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume39
Issue number11
Publication statusPublished - 1991

Fingerprint

lysine decarboxylase
Vibrio parahaemolyticus
Purification
Lysine
Enzymes
Hydroxylysine
Cadaverine
Aminocaproic Acid
Pyridoxal Phosphate
Electrophoresis
Sodium Dodecyl Sulfate
Gel Chromatography
Cysteine
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Molecular Weight
Gels
Molecular weight
Amino Acids
Substrates

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Pharmacology

Cite this

Purification and some properties of inducible lysine decarboxylase from Vibrio parahaemolyticus. / Yamamoto, S.; Imamura, T.; Kusaba, K.; Shinoda, S.

In: Chemical and Pharmaceutical Bulletin, Vol. 39, No. 11, 1991, p. 3067-3070.

Research output: Contribution to journalArticle

Yamamoto, S, Imamura, T, Kusaba, K & Shinoda, S 1991, 'Purification and some properties of inducible lysine decarboxylase from Vibrio parahaemolyticus', Chemical and Pharmaceutical Bulletin, vol. 39, no. 11, pp. 3067-3070.
Yamamoto, S. ; Imamura, T. ; Kusaba, K. ; Shinoda, S. / Purification and some properties of inducible lysine decarboxylase from Vibrio parahaemolyticus. In: Chemical and Pharmaceutical Bulletin. 1991 ; Vol. 39, No. 11. pp. 3067-3070.
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