Purification and some properties of a β-xylosidase and an α-fucosidase from apple snails (pomacea canaliculata)

Koji Hirata, Yuko Nakahara, Yoshinobu Kimura, Gunki Funatsu

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

β-Xylosidase and α-fucosidase were purified from the viscera of apple snails (Pomacea canaliculata) using ammonium sulfate precipitation, hydrophobic chromatography on Butyl-Toyopearl, gel filtration on Sephacryl S-300, and Q-Sepharose column chromatography. 69:85403B2;-Xylosidase and α-fucosidase are glycoproteins and their molecular masses were estimated to be approximately 85 kDa and 54 kDa by SDS-polvacrylamide gel electrophoresis, and assumed to be about 96 kDa and 230 kDa from their sedimentation coefficients, respectively, indicating that the former is a monomer and the latter has a tetrameric structure. β-Xylosidase is stable at pH 4–10 and its optimum pH toward pNP-β-D-xyloside is around 3.6, while α-fucosidase is fairly stable at 65°C and pH 4–10, and its optimum pHs toward pNP-α-L-fucoside are around 3.2 and 5.2. β-Xylosidase released a xylose residue from Xylβ1 → 2Manβ1 → 4GlcNAcβ1 → 4(Fucα1 → 3)GlcNAc-PA and Manα1 → 6(Manα1 → 3)(Xylβ1 → 2)Manβ1 → 4GlcNAcβl → 4(Fucαl → 3)GlcNAc-PA, but not from GlcNAcβl → 2Manα1 → 6(GlcNAcβ1 → 2Manα1 → 3)(Xylβ1 → 2)Manβ1 → 4GlcNAcβ1 → 4(Fucα1 → 3)GlcNAc-PA, while α-fucosidase released a fucose residue from these three PA-oligosaccharides.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume60
Issue number2
DOIs
Publication statusPublished - Jan 1 1996

Keywords

  • Apple snail
  • Exoglycosidase
  • Oligosaccharide structure
  • α-fucosidase
  • β-xylosidase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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