Purification and Some Properties of a β-Xylosidase and an α-Fucosidase from Apple Snails (Pomacea canaliculata)

Koji Hirata, Yuko Nakahara, Yoshinobu Kimura, Gunki Funatsu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

β-Xylosidase and α-fucosidase were purified from the viscera of apple snails (Pomacea canaliculata) using ammonium sulfate precipitation, hydrophobic chromatography on Butyl-Toyopearl, gel filtration on Sephacryl S-300, and Q-Sepharose column chromatography. β-Xylosidase and α-fucosidase are glycoproteins and their molecular masses were estimated to be approximately 85 kDa and 54 kDa by SDS-polyacrylamide gel electrophoresis, and assumed to be about 96 kDa and 230 kDa from their sedimentation coefficients, respectively, indicating that the former is a monomer and the latter has a tetrameric structure. β-Xylosidase is stable at pH 4-10 and its optimum pH toward pNP-β-D-xyloside is around 3.6, while α-fucosidase is fairly stable at 65°C and pH 4-10, and its optimum pHs toward pNP-α-L-fucoside are around 3.2 and 5.2. β-Xylosidase released a xylose residue from Xylβ1→2Manβ1→4GlcNAcβ1→4(Fucα1→3) GlcNAc-PA and Manα1→6(Manα1→3)(Xylβ1→2) Manβ1→4GlcNAcβ1→4(Fucα1→3)GlcNAe-PA, but not from GlcNAcβ1→2Manα1→6(GlcNAcβ1→2Manα1→3) (Xylβ1→2)Manβ1→4GlcNAcβ1→4(Fucα1→ 3)GlcNAc-PA, while α-fucosidase released a fucose residue from these three PA-oligosaccharides.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume60
Issue number2
Publication statusPublished - Feb 1996

Fingerprint

Xylosidases
alpha-L-Fucosidase
Ampullariidae
Pomacea canaliculata
Snails
Malus
Purification
Hydrophobic chromatography
chromatography
Agarose Chromatography
Column chromatography
Fucose
fucose
Viscera
Xylose
Ammonium Sulfate
animal organs
Molecular mass
Electrophoresis
xylose

Keywords

  • α-fucosidase
  • β-xylosidase
  • Apple snail
  • Exoglycosidase
  • Oligosaccharide structure

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

Cite this

Purification and Some Properties of a β-Xylosidase and an α-Fucosidase from Apple Snails (Pomacea canaliculata). / Hirata, Koji; Nakahara, Yuko; Kimura, Yoshinobu; Funatsu, Gunki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 60, No. 2, 02.1996, p. 249-254.

Research output: Contribution to journalArticle

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