We purified to homogeneity an alanine racemase (EC 22.214.171.124) from Thermus thermophilus HB8, an extreme thermophile. Interestingly, the enzyme possessed a monomeric structure with a molecular weight of about 38,000. The enzyme was most active at pH 8 and 75°C, and remained active after incubation at 80°C for 30 min.
- Alanine racemase
- Extreme thermophile
- Thermus thermophilus
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology