Purification and properties of Saccharomyces cerevisiae cystathionine β- synthase

B. I. Ono, K. Kijima, T. Inoue, Shin-ichi Miyoshi, A. Matsuda, S. Shinoda

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Cystathionine β-synthase (β-CTSase), which catalyses cystathionine synthesis from serine and homocysteine, was purified to homogeneity from Saccharomyces cerevisiae. The molecular mass of the enzyme was estimated to be 235 kDa by gel filtration and 55 kDa by sodium dodecyl sulphate- polyacrylamide gel electrophoresis, indicating that it is a homotetramer. The N-terminal amino acid sequence of the enzyme perfectly coincided with that deduced from the nucleotide sequence of CYS4, except for the absence of initiation methionine. The purified β-CTSase catalysed cysteine synthesis from serine (or O-acetylserine) and H2S. From this finding, we discuss the multifunctional nature and evolutionary divergence of S-metabolizing enzymes.

Original languageEnglish
Pages (from-to)333-339
Number of pages7
JournalYeast
Volume10
Issue number3
Publication statusPublished - 1994

Fingerprint

Cystathionine
cystathionine
Yeast
Purification
Saccharomyces cerevisiae
Enzymes
serine
Serine
Gels
enzymes
synthesis
divergent evolution
homocysteine
Sodium dodecyl sulfate
Molecular mass
Homocysteine
Nucleotides
Polyacrylates
Electrophoresis
Sodium Dodecyl Sulfate

Keywords

  • β-synthase
  • amino acid sequence analysis
  • catalytic properties
  • Cystathionine
  • enzyme purification
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Ono, B. I., Kijima, K., Inoue, T., Miyoshi, S., Matsuda, A., & Shinoda, S. (1994). Purification and properties of Saccharomyces cerevisiae cystathionine β- synthase. Yeast, 10(3), 333-339.

Purification and properties of Saccharomyces cerevisiae cystathionine β- synthase. / Ono, B. I.; Kijima, K.; Inoue, T.; Miyoshi, Shin-ichi; Matsuda, A.; Shinoda, S.

In: Yeast, Vol. 10, No. 3, 1994, p. 333-339.

Research output: Contribution to journalArticle

Ono, BI, Kijima, K, Inoue, T, Miyoshi, S, Matsuda, A & Shinoda, S 1994, 'Purification and properties of Saccharomyces cerevisiae cystathionine β- synthase', Yeast, vol. 10, no. 3, pp. 333-339.
Ono BI, Kijima K, Inoue T, Miyoshi S, Matsuda A, Shinoda S. Purification and properties of Saccharomyces cerevisiae cystathionine β- synthase. Yeast. 1994;10(3):333-339.
Ono, B. I. ; Kijima, K. ; Inoue, T. ; Miyoshi, Shin-ichi ; Matsuda, A. ; Shinoda, S. / Purification and properties of Saccharomyces cerevisiae cystathionine β- synthase. In: Yeast. 1994 ; Vol. 10, No. 3. pp. 333-339.
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