TY - JOUR
T1 - Purification and partial sequence analysis of human interleukin-2 derived from peripheral blood leukocytes
AU - Kato, Koichi
AU - Naruo, Ken ichi
AU - Koyama, Masaru
AU - Kawahara, Kenji
AU - Hinuma, Shuji
AU - Tada, Hiroko
AU - Sugino, Hiromu
AU - Tsukamoto, Kyozo
PY - 1985/2/28
Y1 - 1985/2/28
N2 - Human peripheral blood leukocyte-derived interleukin-2 (IL-2) was resolved by DEAE-cellulose column chromatography into three peaks of activity, IL-2A, B, and C, with isoelectric points of 7.2, 6.6, and 7.9, respectively. IL-2 A, B, and C were further purified by reverse phase high performance liquid chromatography and resolved into two apparently homogeneous peaks each with identical molecular weight: A-1 and A-2 (Mr17000); B-1 and B-2 (Mr17500); and C-1 and C-2 (Mr14400). The amino acid compositions and partial NH2-terminal amino acid sequences of these molecular species were consistent with those predicted from IL-2 cDNA sequences derived from Jurkat and peripheral blood leukocytes.
AB - Human peripheral blood leukocyte-derived interleukin-2 (IL-2) was resolved by DEAE-cellulose column chromatography into three peaks of activity, IL-2A, B, and C, with isoelectric points of 7.2, 6.6, and 7.9, respectively. IL-2 A, B, and C were further purified by reverse phase high performance liquid chromatography and resolved into two apparently homogeneous peaks each with identical molecular weight: A-1 and A-2 (Mr17000); B-1 and B-2 (Mr17500); and C-1 and C-2 (Mr14400). The amino acid compositions and partial NH2-terminal amino acid sequences of these molecular species were consistent with those predicted from IL-2 cDNA sequences derived from Jurkat and peripheral blood leukocytes.
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U2 - 10.1016/S0006-291X(85)80142-X
DO - 10.1016/S0006-291X(85)80142-X
M3 - Article
C2 - 3872125
AN - SCOPUS:0022004031
VL - 127
SP - 182
EP - 190
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -