Purification and partial sequence analysis of human interleukin-2 derived from peripheral blood leukocytes

Koichi Kato; Ken ichi Naruo; Masaru Koyama; Kenji Kawahara; Shuji Hinuma; Hiroko Tada; Hiromu Sugino; Kyozo Tsukamoto

doi:10.1016/S0006-291X(85)80142-X

Purification and partial sequence analysis of human interleukin-2 derived from peripheral blood leukocytes

Koichi Kato, Ken ichi Naruo, Masaru Koyama, Kenji Kawahara, Shuji Hinuma, Hiroko Tada, Hiromu Sugino, Kyozo Tsukamoto

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Human peripheral blood leukocyte-derived interleukin-2 (IL-2) was resolved by DEAE-cellulose column chromatography into three peaks of activity, IL-2A, B, and C, with isoelectric points of 7.2, 6.6, and 7.9, respectively. IL-2 A, B, and C were further purified by reverse phase high performance liquid chromatography and resolved into two apparently homogeneous peaks each with identical molecular weight: A-1 and A-2 (Mr17000); B-1 and B-2 (Mr17500); and C-1 and C-2 (Mr14400). The amino acid compositions and partial NH₂-terminal amino acid sequences of these molecular species were consistent with those predicted from IL-2 cDNA sequences derived from Jurkat and peripheral blood leukocytes.

Original language	English
Pages (from-to)	182-190
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	127
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(85)80142-X
Publication status	Published - Feb 28 1985
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Purification and partial sequence analysis of human interleukin-2 derived from peripheral blood leukocytes. / Kato, Koichi; Naruo, Ken ichi; Koyama, Masaru; Kawahara, Kenji; Hinuma, Shuji; Tada, Hiroko; Sugino, Hiromu; Tsukamoto, Kyozo.

In: Biochemical and Biophysical Research Communications, Vol. 127, No. 1, 28.02.1985, p. 182-190.

Research output: Contribution to journal › Article › peer-review

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abstract = "Human peripheral blood leukocyte-derived interleukin-2 (IL-2) was resolved by DEAE-cellulose column chromatography into three peaks of activity, IL-2A, B, and C, with isoelectric points of 7.2, 6.6, and 7.9, respectively. IL-2 A, B, and C were further purified by reverse phase high performance liquid chromatography and resolved into two apparently homogeneous peaks each with identical molecular weight: A-1 and A-2 (Mr17000); B-1 and B-2 (Mr17500); and C-1 and C-2 (Mr14400). The amino acid compositions and partial NH2-terminal amino acid sequences of these molecular species were consistent with those predicted from IL-2 cDNA sequences derived from Jurkat and peripheral blood leukocytes.",

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AU - Kato, Koichi

AU - Naruo, Ken ichi

AU - Koyama, Masaru

AU - Kawahara, Kenji

AU - Hinuma, Shuji

AU - Tada, Hiroko

AU - Sugino, Hiromu

AU - Tsukamoto, Kyozo

PY - 1985/2/28

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N2 - Human peripheral blood leukocyte-derived interleukin-2 (IL-2) was resolved by DEAE-cellulose column chromatography into three peaks of activity, IL-2A, B, and C, with isoelectric points of 7.2, 6.6, and 7.9, respectively. IL-2 A, B, and C were further purified by reverse phase high performance liquid chromatography and resolved into two apparently homogeneous peaks each with identical molecular weight: A-1 and A-2 (Mr17000); B-1 and B-2 (Mr17500); and C-1 and C-2 (Mr14400). The amino acid compositions and partial NH2-terminal amino acid sequences of these molecular species were consistent with those predicted from IL-2 cDNA sequences derived from Jurkat and peripheral blood leukocytes.

AB - Human peripheral blood leukocyte-derived interleukin-2 (IL-2) was resolved by DEAE-cellulose column chromatography into three peaks of activity, IL-2A, B, and C, with isoelectric points of 7.2, 6.6, and 7.9, respectively. IL-2 A, B, and C were further purified by reverse phase high performance liquid chromatography and resolved into two apparently homogeneous peaks each with identical molecular weight: A-1 and A-2 (Mr17000); B-1 and B-2 (Mr17500); and C-1 and C-2 (Mr14400). The amino acid compositions and partial NH2-terminal amino acid sequences of these molecular species were consistent with those predicted from IL-2 cDNA sequences derived from Jurkat and peripheral blood leukocytes.

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Purification and partial sequence analysis of human interleukin-2 derived from peripheral blood leukocytes

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