Purification and partial characterization of histamine N-methyltransferase from bovine brain

Masahiro Nishibori, Ryozo Oishi, Yoshinori Itoh, Kiyomi Saeki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

S-Adenosylmethionine:histamine N-methyltransferase (EC 2.1.1.8.) (HMT) catalyzes the transfer of a methyl group from S-adenosyl-l-methionine to histamine resulting in the formation of tele-methylhistamine and S-adenosyl-l-homocysteine. In the present study, we purified HMT from bovine brain since this enzyme has been suggested to have some different kinetic properties from that from mouse or rat brain. The overall purification was about 8000-fold with a 2.9% yield. The specific activity of the final preparation was 517 nmol/min/mg protein. The pivotal steps in the purification consisted of ammonium sulfate precipitation, linear gradient DEAE-cellulose chromatography and elution with histamine and S-adenosylmethionine in DEAE cellulose chromatography. Bovine brain HMT could not be retained on the affinity columns using S-adenosylhomocysteine and histamine as ligands under the conditions tested. Molecular weight of the purified enzyme was determined to be 33,200 dalton by SDS-polyacrylamide gel electrophoresis. A pI value of 5.9 was obtained by isoelectric focusing. Amodiaquine and metoprine competitively inhibited the enzyme activity with respect to histamine with Ki values of 7.5 and 58 nM, respectively.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalNeurochemistry International
Volume19
Issue number1-2
DOIs
Publication statusPublished - 1991

Fingerprint

Histamine N-Methyltransferase
Histamine
S-Adenosylmethionine
DEAE-Cellulose Chromatography
Brain
Enzymes
S-Adenosylhomocysteine
Amodiaquine
Ammonium Sulfate
Isoelectric Focusing
Homocysteine
Methionine
Polyacrylamide Gel Electrophoresis
Molecular Weight
Ligands
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Purification and partial characterization of histamine N-methyltransferase from bovine brain. / Nishibori, Masahiro; Oishi, Ryozo; Itoh, Yoshinori; Saeki, Kiyomi.

In: Neurochemistry International, Vol. 19, No. 1-2, 1991, p. 135-141.

Research output: Contribution to journalArticle

Nishibori, Masahiro ; Oishi, Ryozo ; Itoh, Yoshinori ; Saeki, Kiyomi. / Purification and partial characterization of histamine N-methyltransferase from bovine brain. In: Neurochemistry International. 1991 ; Vol. 19, No. 1-2. pp. 135-141.
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