Purification and partial bioactivity in vitro of polysaccharides from sporophyll of Undaria pinnatifida

Hang Qi, Yangding Yu, Liming Sun, Jingfeng Yang, Dongmei Li, Chi Xin, Dayong Zhou, Lei Qin, Yoshiyuki Murata

Research output: Contribution to journalArticle

Abstract

In China, sporophyll of brown seaweeds, Undaria pinnatifida, as processing waste was discarded, that caused a great waste of resource and induced environment pollution. Polysaccharide from the sporophyll of Undaria pinnatifida (PSU) is interesting due to its various biological activities. This polysaccharide was isolated from raw material by dilute acid extraction and crude extract was purified by chromatography on DEAE-cellulose. Four fractions of PSU were obtained and the composition of the four fractions of PSU was characterized by organic elemental analysis and GC. Comparing the other fractions, PSU I contained the most protein and uronic acid and PSU III the most carbohydrate and sulfate. Fucose, galactose and mannose were contained in all of fractions, but rhamnose and glucose were contained only in PSU I. The ratio of galactose and fucose in PSU IV (0.88:1) and PSU III (0.32:1) was higher than in the other fractions, which was the critical factor to its anti-inflamatory and antioxidant activities. Biological activity research in vitro showed that PSU IV could inhibit RAW 264.7 cell, releasing NO induced by LPS. PSU IV had a strong resistance to cell oxidative damage caused by tBOOH and all of the fractions.

Original languageEnglish
Pages (from-to)197-201
Number of pages5
JournalJournal of Food, Agriculture and Environment
Volume10
Issue number3-4
Publication statusPublished - 2012

Fingerprint

Undaria
Undaria pinnatifida
bioactivity
Fucose
fucose
Galactose
polysaccharide
galactose
Polysaccharides
purification
bioactive properties
polysaccharides
processing waste
Uronic Acids
Seaweed
Rhamnose
DEAE-Cellulose Chromatography
Phaeophyceae
uronic acids
rhamnose

Keywords

  • Anti-inflamatory activity
  • Antioxidant activity
  • Lymphocyte proliferation
  • Polysaccharide
  • Purification
  • Sporophyll
  • Undaria pinnatifida (PSU)

ASJC Scopus subject areas

  • Food Science
  • Agricultural and Biological Sciences (miscellaneous)
  • Environmental Science(all)

Cite this

Purification and partial bioactivity in vitro of polysaccharides from sporophyll of Undaria pinnatifida. / Qi, Hang; Yu, Yangding; Sun, Liming; Yang, Jingfeng; Li, Dongmei; Xin, Chi; Zhou, Dayong; Qin, Lei; Murata, Yoshiyuki.

In: Journal of Food, Agriculture and Environment, Vol. 10, No. 3-4, 2012, p. 197-201.

Research output: Contribution to journalArticle

Qi, Hang ; Yu, Yangding ; Sun, Liming ; Yang, Jingfeng ; Li, Dongmei ; Xin, Chi ; Zhou, Dayong ; Qin, Lei ; Murata, Yoshiyuki. / Purification and partial bioactivity in vitro of polysaccharides from sporophyll of Undaria pinnatifida. In: Journal of Food, Agriculture and Environment. 2012 ; Vol. 10, No. 3-4. pp. 197-201.
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AU - Xin, Chi

AU - Zhou, Dayong

AU - Qin, Lei

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