Purification and characterization of sulfide:quinone oxidoreductase from an acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans

Satoshi Wakai, Mizuho Tsujita, Mei Kikumoto, Mohammed A. Manchur, Tadayoshi Kanao, Kazuo Kamimura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Sulfide:quinone oxidoreductase (SQR) was purified from membrane of acidophilic chemolithotrophic bacterium Acidithiobacillus ferrooxidans NASF-1 cells grown on sulfur medium. It was composed of a single polypeptide with an apparent molecular mass of 47 kDa. The apparent Km values for sulfide and ubiquinone were 42 and 14 μM respectively. The apparent optimum pH for the SQR activity was about 7.0. A gene encoding a putative SQR of A. ferrooxidans NASF-1 was cloned and sequenced. The gene was expressed in Escherichia coli as a thioredoxin-fusion protein in inclusion bodies in an inactive form. A polyclonal antibody prepared against the recombinant protein reacted immunologically with the purified SQR. Western blotting analysis using the antibody revealed an increased level of SQR synthesis in sulfur-grown A. ferrooxidans NASF-1 cells, implying the involvement of SQR in elemental sulfur oxidation in sulfur-grown A. ferrooxidans NASF-1 cells.

Original languageEnglish
Pages (from-to)2735-2742
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number11
DOIs
Publication statusPublished - 2007

Keywords

  • Acidithiobacillus ferrooxidans
  • Acidophile
  • Sulfide:quinone oxidoreductase
  • Sulfur oxidation

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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