TY - JOUR
T1 - Purification and Characterization of Membrane Protein (90 kDa) from Mycoplasma salivarium, Which Binds Immunoglobulin (Ig) G Fc Fragment
AU - Sawa, Yoshihiko
AU - Shibata, Ken Ichiro
AU - Noda, Mamoru
AU - Watanabe, Tsuguo
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1994
Y1 - 1994
N2 - A 90 kDa protein of Mycoplasma salivarium was released from cell membranes of the organism with Triton X-100 and purified by ion-exchange chromatography and chromatofocusing. The protein was eluted at pH 5.5 by chromatofocusing. The protein was shown to react with the Fc fragments of IgG from human and nine different animal species and did not distinguish between IgG from different species, while protein A, tested for comparative purposes, displayed a strong specificity for human and swine IgG. Furthermore, the protein reacted with antigen specific goat IgG (specific for gamma chains of human IgG), sheep red blood cells (SRBC) sensitized with rabbit antiserum to SRBC, that is, the Fc part of rabbit IgG, and concanavalin A as well. These findings may suggest that the protein is a lectin which binds the carbohydrate moiety of the Fc part of IgG.
AB - A 90 kDa protein of Mycoplasma salivarium was released from cell membranes of the organism with Triton X-100 and purified by ion-exchange chromatography and chromatofocusing. The protein was eluted at pH 5.5 by chromatofocusing. The protein was shown to react with the Fc fragments of IgG from human and nine different animal species and did not distinguish between IgG from different species, while protein A, tested for comparative purposes, displayed a strong specificity for human and swine IgG. Furthermore, the protein reacted with antigen specific goat IgG (specific for gamma chains of human IgG), sheep red blood cells (SRBC) sensitized with rabbit antiserum to SRBC, that is, the Fc part of rabbit IgG, and concanavalin A as well. These findings may suggest that the protein is a lectin which binds the carbohydrate moiety of the Fc part of IgG.
KW - IgG Fc-binding protein
KW - Mycoplasma salivarium
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U2 - 10.1111/j.1348-0421.1994.tb01773.x
DO - 10.1111/j.1348-0421.1994.tb01773.x
M3 - Article
C2 - 7523837
AN - SCOPUS:0028281350
VL - 38
SP - 257
EP - 262
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 4
ER -