Purification and characterization of L-2,4-diaminobutyrate decarboxylase from Acinetobacter calcoaceticus

S. Yamamoto, Y. Tsuzaki, K. Tougou, S. Shinoda

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Abstract

Acinetobacter calcoaceticus ATCC 23055 produces a large amount of 1,3-diaminopropane under normal growth conditions. The enzyme responsible, L-2,4-diaminobutyrate (DABA) decarboxylase (EC 4.1.1.-), was purified to electrophoretic homogeneity from this bacterium. The native enzyme had an M(r) of approximately 108000, with a pI of 5.0, and was a dimer composed of identical or nearly identical subunits with apparent M(r) 53000. The enzyme showed hyperbolic kinetics with a K(m) of 1.59mM for DABA and 14.6μM for pyridoxal 5'-phosphate as a coenzyme. The pH optimum was in the range 8.5-8.75, and Ca2+ gave a much higher enzyme activity than Mg2+ as a cationic cofactor. N-γ-AcetylDABA, 2,3-diaminopropionic acid, ornithine and lysine were inert as substrates. The enzyme was different in subunit structure, N-terminal amino acid sequence and immunoreactivity from the DABA decarboxylase of Vibrio alginolyticus previously described.

Original languageEnglish
Pages (from-to)1461-1465
Number of pages5
JournalJournal of General Microbiology
Volume138
Issue number7
Publication statusPublished - 1992

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Acinetobacter calcoaceticus
Enzymes
Vibrio alginolyticus
Pyridoxal Phosphate
Ornithine
Carboxy-Lyases
Coenzymes
Lysine
Amino Acid Sequence
2,4-diaminobutyrate decarboxylase
Bacteria
Growth

ASJC Scopus subject areas

  • Microbiology

Cite this

Purification and characterization of L-2,4-diaminobutyrate decarboxylase from Acinetobacter calcoaceticus. / Yamamoto, S.; Tsuzaki, Y.; Tougou, K.; Shinoda, S.

In: Journal of General Microbiology, Vol. 138, No. 7, 1992, p. 1461-1465.

Research output: Contribution to journalArticle

Yamamoto, S, Tsuzaki, Y, Tougou, K & Shinoda, S 1992, 'Purification and characterization of L-2,4-diaminobutyrate decarboxylase from Acinetobacter calcoaceticus', Journal of General Microbiology, vol. 138, no. 7, pp. 1461-1465.
Yamamoto, S. ; Tsuzaki, Y. ; Tougou, K. ; Shinoda, S. / Purification and characterization of L-2,4-diaminobutyrate decarboxylase from Acinetobacter calcoaceticus. In: Journal of General Microbiology. 1992 ; Vol. 138, No. 7. pp. 1461-1465.
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