Purification and characterization of dog liver microsomal epoxide hydrolase

Noritaka Ariyoshi, Mitsuko Tanaka, Yuji Ishii, Kazuta Oguri

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An epoxide hydrolase (mEH) in liver microsomes was purified to apparent homogeneity from a dog treated with phenobarbital. The purified enzyme had a minimum molecular weight of 47,000 as determined by SDS-PAGE. The dog mEH activity was characterized by use of a substrate, 7-glycidoxycoumarin (GOC), and some effectors of this enzyme. In vitro activators, metyrapone, and isoquinoline, stimulated the microsomal activity, but the former had no such effect on the purified enzyme in case of this substrate. All mEH inhibitors, 1,1,1-trichloropropene 2,3-oxide (TCPO), cyclohexene oxide, and 2-bromo-4'-nitroacetophenone (BrNAP), suppressed hydrolase activity. The NH2-terminal amino acid sequence of the purified enzyme was highly homologous (90%) to the sequences deduced from a cDNA clone of rat enzyme. Antiserum to the purified enzyme raised in rabbits cross-reacted with rat and Guinea pig epoxide hydrolases. No gender-difference in this enzyme in liver microsomes was observed in dogs.

Original languageEnglish
Pages (from-to)985-990
Number of pages6
JournalJournal of Biochemistry
Volume115
Issue number5
Publication statusPublished - May 1994
Externally publishedYes

Fingerprint

Epoxide Hydrolases
Hydrolases
Purification
Liver
Enzymes
Dogs
Liver Microsomes
Oxides
Rats
Trichloroepoxypropane
Substrate
Gender Differences
Metyrapone
Guinea pig
Substrates
Rabbit
Phenobarbital
Amino Acid Sequence
CDNA
Dog

Keywords

  • Dog
  • Microsomal epoxide hydrolase
  • Purification

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Purification and characterization of dog liver microsomal epoxide hydrolase. / Ariyoshi, Noritaka; Tanaka, Mitsuko; Ishii, Yuji; Oguri, Kazuta.

In: Journal of Biochemistry, Vol. 115, No. 5, 05.1994, p. 985-990.

Research output: Contribution to journalArticle

Ariyoshi, N, Tanaka, M, Ishii, Y & Oguri, K 1994, 'Purification and characterization of dog liver microsomal epoxide hydrolase', Journal of Biochemistry, vol. 115, no. 5, pp. 985-990.
Ariyoshi, Noritaka ; Tanaka, Mitsuko ; Ishii, Yuji ; Oguri, Kazuta. / Purification and characterization of dog liver microsomal epoxide hydrolase. In: Journal of Biochemistry. 1994 ; Vol. 115, No. 5. pp. 985-990.
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