Purification and Characterization of an Acid Phosphatase from Mycoplasma fermentans

Mamoru Noda, Ken Ichiro Shibata, Yoshihiko Sawa, Tsuguo Watanabe, Hirokata Shimokoube

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An acid phosphatase associated with the cell membranes of Mycoplasma fermentans was released from the membranes with Triton X-100, then purified by ion-exchange chromatography on DEAE-Sephacel and CM-Sepharose, followed by affinity chromatography on Con A-Sepharose. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme revealed a single band with a molecular mass of 31.2 kilodaltons. The enzyme activity toward p-nitrophenyl phosphate was enhanced remarkably by Cu2+, Co2+ and Mg2+, but the activity was not inhibited by EDTA. The enzyme dephosphorylated 0-phospho-L-tyrosine as well as p-nitrophenyl phosphate, but not O-phospho-L-threonine, O-phospho-L-serine, glucose-l-phosphate, phosphoryl choline and adenosine triphosphate. The level of the 0-phospho-L-tyrosine phosphatase activity was the highest in Mycoplasma faucium and the second highest in Mycoplasma fermentans of all tested human mycoplasmas.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalMicrobiology and Immunology
Volume38
Issue number2
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Mycoplasma fermentans
Acid Phosphatase
Mycoplasma
Tyrosine
Enzymes
Phosphorylcholine
Ion Exchange Chromatography
Octoxynol
Threonine
Affinity Chromatography
Phosphoric Monoester Hydrolases
Edetic Acid
Sodium Dodecyl Sulfate
Sepharose
Serine
Polyacrylamide Gel Electrophoresis
Adenosine Triphosphate
Cell Membrane
Glucose
Membranes

Keywords

  • Acid phosphatase
  • Mycoplasma fermentans

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Noda, M., Shibata, K. I., Sawa, Y., Watanabe, T., & Shimokoube, H. (1994). Purification and Characterization of an Acid Phosphatase from Mycoplasma fermentans. Microbiology and Immunology, 38(2), 103-107.

Purification and Characterization of an Acid Phosphatase from Mycoplasma fermentans. / Noda, Mamoru; Shibata, Ken Ichiro; Sawa, Yoshihiko; Watanabe, Tsuguo; Shimokoube, Hirokata.

In: Microbiology and Immunology, Vol. 38, No. 2, 1994, p. 103-107.

Research output: Contribution to journalArticle

Noda, M, Shibata, KI, Sawa, Y, Watanabe, T & Shimokoube, H 1994, 'Purification and Characterization of an Acid Phosphatase from Mycoplasma fermentans', Microbiology and Immunology, vol. 38, no. 2, pp. 103-107.
Noda, Mamoru ; Shibata, Ken Ichiro ; Sawa, Yoshihiko ; Watanabe, Tsuguo ; Shimokoube, Hirokata. / Purification and Characterization of an Acid Phosphatase from Mycoplasma fermentans. In: Microbiology and Immunology. 1994 ; Vol. 38, No. 2. pp. 103-107.
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