Purification and characterization of a membrane-bound atpase from acetabularia cliftonii that corresponds to a cl--Translocating ATPase in A cetabularia acetabulum

Chie Moritani, Toshitaka Oohashi, Sachiko Satoh, Dieter Oesterhelt, Mikiko Ikeda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A Mg2+-ATPase was solubilized from membranes of Acetabularia cliftonii using nonanoyl-N-methylgluconamide and purified by ion-exchange and gel permeation chromatography. One active ATPase fraction after Mono Q chromatography had a specific activity of 10 units/mg of protein. Judged from subunit composition [54 (a), 50 (b) with a fainter band around 40 kDa], catalytic properties, and N-terminal amino acid sequence of the b subunit, the isolated enzyme was comparable to the Cl-ATPase of Acetabularia acetabulum. Immunological characterization of both subunits showed significant similarity to the F type of ATPase. Cl-transport activity was observed by reconstitution studies into liposomes.

Original languageEnglish
Pages (from-to)2087-2089
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume58
Issue number11
DOIs
Publication statusPublished - 1994

Fingerprint

Acetabularia
Acetabulum
Purification
adenosinetriphosphatase
Adenosine Triphosphatases
Membranes
Ca(2+) Mg(2+)-ATPase
Ion Exchange
Gel permeation chromatography
Chromatography
Liposomes
Gel Chromatography
Amino Acid Sequence
Ion exchange
Amino Acids
ion exchange
protein subunits
Enzymes
Chemical analysis
chromatography

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Organic Chemistry
  • Analytical Chemistry
  • Applied Microbiology and Biotechnology
  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)

Cite this

Purification and characterization of a membrane-bound atpase from acetabularia cliftonii that corresponds to a cl--Translocating ATPase in A cetabularia acetabulum. / Moritani, Chie; Oohashi, Toshitaka; Satoh, Sachiko; Oesterhelt, Dieter; Ikeda, Mikiko.

In: Bioscience, Biotechnology and Biochemistry, Vol. 58, No. 11, 1994, p. 2087-2089.

Research output: Contribution to journalArticle

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