Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients

Yoshinobu Kimura, Megumi Maeda, Mariko Kimura, Ming Lai Oi, Hee Tan Siang, Mei Hon Sook, Tim Chew Fook

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A basic glycoprotein, which was recognized by IgE from oil palm pollinosis patients, has been purified from oil palm pollen (Elaeis guineensis Jacq.), which is a strong allergen and causes severe pollinosis in Malaysia and Singapore. Soluble proteins were extracted from defatted palm pollen with both Tris-HCl buffer (pH 7.8) and Na-acetate buffer (pH 4.0). The allergenic glycoprotein was purified from the total extract to homogeneity with 0.4% yield by a combination of DEAE- and CM-cellulose, SP-HPLC, and gel filtration. The purified oil palm pollen glycoprotein with molecular mass of 31 kDa was recognized by the β1-2 xylose specific antibody, suggesting this basic glycoprotein bears plant complex type N-glycan(s). The palm pollen basic glycoprotein, designated Ela g Bd 31K, was recognized by IgE of palm pollinosis patients, suggesting Ela g Bd 31 K should be one of the palm pollen allergens. The preliminary structural analysis of N-glycans linked to glycoproteins of palm pollens showed that the antigenic N-glycans having α1-3 fucose and β1-2 xylose residues (GlcNAc2-0Man3Xyl 1Fuc1-0GlcNAc2) actually occur on the palm pollen glycoproteins, in addition to the high-mannose type structures (Man 9-5 GlcNAc2).

Original languageEnglish
Pages (from-to)820-827
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number4
Publication statusPublished - Apr 2002

Fingerprint

hay fever
Glycoproteins
Seasonal Allergic Rhinitis
Pollen
Immunoglobulin E
Purification
glycoproteins
pollen
Elaeis guineensis
Palm oil
Allergens
Polysaccharides
Oils
Xylose
polysaccharides
allergens
xylose
Buffers
buffers
DEAE-Cellulose

Keywords

  • Antigenic N-glycan
  • Elaeis guineensis Jacq.
  • Oil palm pollen allergen
  • Plant glycoprotein
  • Pollen allergy

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients. / Kimura, Yoshinobu; Maeda, Megumi; Kimura, Mariko; Oi, Ming Lai; Siang, Hee Tan; Sook, Mei Hon; Fook, Tim Chew.

In: Bioscience, Biotechnology and Biochemistry, Vol. 66, No. 4, 04.2002, p. 820-827.

Research output: Contribution to journalArticle

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