Purification and characterization of 3-isopropylmalate dehydrogenase from Thiobacillus thiooxidans

Hiroshi Kawaguchi, Kenji Inagaki, Hideyuki Matsunami, Yumi Nakayama, Tatsuo Tano, Hidehiko Tanaka

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

3-Isopropylmalate dehydrogenase was purified to homogeneity from the acidophilic autotroph Thiobacillus thiooxidans. The native enzyme was a dimer of molecular weight 40,000. The apparent K(m) values for 3-isopropylmalate and NAD+ were estimated to be 0.13 mM and 8.7 mM, respectively. The optimum pH for activity was 9.0 and the optimum temperature was 65°C. The properties of the enzyme were similar to those of the Thiobacillus ferrooxidans enzyme, expect for substrate specificity. T. thiooxidans 3-isopropylmalate dehydrogenase could not utilize malate as a substrate.

Original languageEnglish
Pages (from-to)459-461
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume90
Issue number4
DOIs
Publication statusPublished - Oct 2000

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3-Isopropylmalate Dehydrogenase
Acidithiobacillus thiooxidans
Purification
Enzymes
Thiobacillus
Substrates
Substrate Specificity
Dimers
NAD
Molecular Weight
Molecular weight
Temperature
Oxidoreductases

Keywords

  • 3-isopropylmalate dehydrogenase
  • Purification
  • Thiobacillus thiooxidans

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Purification and characterization of 3-isopropylmalate dehydrogenase from Thiobacillus thiooxidans. / Kawaguchi, Hiroshi; Inagaki, Kenji; Matsunami, Hideyuki; Nakayama, Yumi; Tano, Tatsuo; Tanaka, Hidehiko.

In: Journal of Bioscience and Bioengineering, Vol. 90, No. 4, 10.2000, p. 459-461.

Research output: Contribution to journal › Article

Kawaguchi, H, Inagaki, K, Matsunami, H, Nakayama, Y, Tano, T & Tanaka, H 2000, 'Purification and characterization of 3-isopropylmalate dehydrogenase from Thiobacillus thiooxidans', Journal of Bioscience and Bioengineering, vol. 90, no. 4, pp. 459-461. https://doi.org/10.1016/S1389-1723(01)80020-7
Kawaguchi H, Inagaki K, Matsunami H, Nakayama Y, Tano T, Tanaka H. Purification and characterization of 3-isopropylmalate dehydrogenase from Thiobacillus thiooxidans. Journal of Bioscience and Bioengineering. 2000 Oct;90(4):459-461. https://doi.org/10.1016/S1389-1723(01)80020-7
Kawaguchi, Hiroshi ; Inagaki, Kenji ; Matsunami, Hideyuki ; Nakayama, Yumi ; Tano, Tatsuo ; Tanaka, Hidehiko. / Purification and characterization of 3-isopropylmalate dehydrogenase from Thiobacillus thiooxidans. In: Journal of Bioscience and Bioengineering. 2000 ; Vol. 90, No. 4. pp. 459-461.
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