Purification and cDNA cloning of the ovigerous-hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir

Oleg Gusev, Hideki Ikeda, Tetsushi Okochi, Jae Min Lee, Masatsugu Hatakeyama, Chiyoko Kobayashi, Kiyokazu Agata, Hidenori Yamada, Masayuki Saigusa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The egg attachment system of an estuarine crab Sesarma haematocheir is formed on the maternal ovigerous hairs just after egg laying, and slips off these hairs just after hatching. The stripping is caused by an active factor that we call OHSS (ovigerous-hair stripping substance), which is released by the embryo upon hatching. OHSS was purified, and its active form had a molecular mass of 25 kDa. The cDNA of OHSS cloned from an embryonic cDNA library was 1759 bp long, encoding 492 amino acids in a single open reading frame (ORF). The C-terminal part of the predicted protein was composed of a trypsin-like serine protease domain, with homology to counterparts in other animals of 33-38%. The predicted protein (54.7 kDa) secreted as a zymogen may be cleaved post-translationally, separating the C-terminal from the N-terminal region. The OHSS gene was expressed in the embryo at least 2 weeks before hatching. Expression was also detected in the zoea larva 1 day after hatching and in the brain of the female. However, it was not detected in the muscle, hepatopancreas or ovigerous seta of the female. Ultrastructural analysis indicated that the material investing maternal ovigerous hair, i.e. the outermost layer (E1) of the egg case, is attached at the special sites (attachment sites) arranged at intervals of 130-160 nm on the hair. It is suggested that OHSS acts specifically at these sites, lysing the bond with the coat, thus disposing of the embryo attachment system. This enables the female to prepare the next clutch of embryos without ecdysis.

Original languageEnglish
Pages (from-to)621-632
Number of pages12
JournalJournal of Experimental Biology
Volume207
Issue number4
DOIs
Publication statusPublished - Feb 2004

Fingerprint

brackish water
hair
hairs
purification
Organism Cloning
molecular cloning
crab
crabs
Embryonic Structures
Complementary DNA
Ovum
Water
hatching
embryo
Mothers
embryo (animal)
Hepatopancreas
Sensilla
Molting
water

Keywords

  • Crab
  • Embryo attachment system
  • Investment coat
  • Ovigerous hair
  • Ovigerous-hair stripping substance (OHSS)
  • Serine protease
  • Sesarma (or Chiromantes) haematocheir
  • Stripping

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Purification and cDNA cloning of the ovigerous-hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir. / Gusev, Oleg; Ikeda, Hideki; Okochi, Tetsushi; Lee, Jae Min; Hatakeyama, Masatsugu; Kobayashi, Chiyoko; Agata, Kiyokazu; Yamada, Hidenori; Saigusa, Masayuki.

In: Journal of Experimental Biology, Vol. 207, No. 4, 02.2004, p. 621-632.

Research output: Contribution to journalArticle

Gusev, O, Ikeda, H, Okochi, T, Lee, JM, Hatakeyama, M, Kobayashi, C, Agata, K, Yamada, H & Saigusa, M 2004, 'Purification and cDNA cloning of the ovigerous-hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir', Journal of Experimental Biology, vol. 207, no. 4, pp. 621-632. https://doi.org/10.1242/jeb.00785
Gusev, Oleg ; Ikeda, Hideki ; Okochi, Tetsushi ; Lee, Jae Min ; Hatakeyama, Masatsugu ; Kobayashi, Chiyoko ; Agata, Kiyokazu ; Yamada, Hidenori ; Saigusa, Masayuki. / Purification and cDNA cloning of the ovigerous-hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir. In: Journal of Experimental Biology. 2004 ; Vol. 207, No. 4. pp. 621-632.
@article{6b4dbba79d564e248d7111b7463aa9f2,
title = "Purification and cDNA cloning of the ovigerous-hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir",
abstract = "The egg attachment system of an estuarine crab Sesarma haematocheir is formed on the maternal ovigerous hairs just after egg laying, and slips off these hairs just after hatching. The stripping is caused by an active factor that we call OHSS (ovigerous-hair stripping substance), which is released by the embryo upon hatching. OHSS was purified, and its active form had a molecular mass of 25 kDa. The cDNA of OHSS cloned from an embryonic cDNA library was 1759 bp long, encoding 492 amino acids in a single open reading frame (ORF). The C-terminal part of the predicted protein was composed of a trypsin-like serine protease domain, with homology to counterparts in other animals of 33-38{\%}. The predicted protein (54.7 kDa) secreted as a zymogen may be cleaved post-translationally, separating the C-terminal from the N-terminal region. The OHSS gene was expressed in the embryo at least 2 weeks before hatching. Expression was also detected in the zoea larva 1 day after hatching and in the brain of the female. However, it was not detected in the muscle, hepatopancreas or ovigerous seta of the female. Ultrastructural analysis indicated that the material investing maternal ovigerous hair, i.e. the outermost layer (E1) of the egg case, is attached at the special sites (attachment sites) arranged at intervals of 130-160 nm on the hair. It is suggested that OHSS acts specifically at these sites, lysing the bond with the coat, thus disposing of the embryo attachment system. This enables the female to prepare the next clutch of embryos without ecdysis.",
keywords = "Crab, Embryo attachment system, Investment coat, Ovigerous hair, Ovigerous-hair stripping substance (OHSS), Serine protease, Sesarma (or Chiromantes) haematocheir, Stripping",
author = "Oleg Gusev and Hideki Ikeda and Tetsushi Okochi and Lee, {Jae Min} and Masatsugu Hatakeyama and Chiyoko Kobayashi and Kiyokazu Agata and Hidenori Yamada and Masayuki Saigusa",
year = "2004",
month = "2",
doi = "10.1242/jeb.00785",
language = "English",
volume = "207",
pages = "621--632",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "4",

}

TY - JOUR

T1 - Purification and cDNA cloning of the ovigerous-hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir

AU - Gusev, Oleg

AU - Ikeda, Hideki

AU - Okochi, Tetsushi

AU - Lee, Jae Min

AU - Hatakeyama, Masatsugu

AU - Kobayashi, Chiyoko

AU - Agata, Kiyokazu

AU - Yamada, Hidenori

AU - Saigusa, Masayuki

PY - 2004/2

Y1 - 2004/2

N2 - The egg attachment system of an estuarine crab Sesarma haematocheir is formed on the maternal ovigerous hairs just after egg laying, and slips off these hairs just after hatching. The stripping is caused by an active factor that we call OHSS (ovigerous-hair stripping substance), which is released by the embryo upon hatching. OHSS was purified, and its active form had a molecular mass of 25 kDa. The cDNA of OHSS cloned from an embryonic cDNA library was 1759 bp long, encoding 492 amino acids in a single open reading frame (ORF). The C-terminal part of the predicted protein was composed of a trypsin-like serine protease domain, with homology to counterparts in other animals of 33-38%. The predicted protein (54.7 kDa) secreted as a zymogen may be cleaved post-translationally, separating the C-terminal from the N-terminal region. The OHSS gene was expressed in the embryo at least 2 weeks before hatching. Expression was also detected in the zoea larva 1 day after hatching and in the brain of the female. However, it was not detected in the muscle, hepatopancreas or ovigerous seta of the female. Ultrastructural analysis indicated that the material investing maternal ovigerous hair, i.e. the outermost layer (E1) of the egg case, is attached at the special sites (attachment sites) arranged at intervals of 130-160 nm on the hair. It is suggested that OHSS acts specifically at these sites, lysing the bond with the coat, thus disposing of the embryo attachment system. This enables the female to prepare the next clutch of embryos without ecdysis.

AB - The egg attachment system of an estuarine crab Sesarma haematocheir is formed on the maternal ovigerous hairs just after egg laying, and slips off these hairs just after hatching. The stripping is caused by an active factor that we call OHSS (ovigerous-hair stripping substance), which is released by the embryo upon hatching. OHSS was purified, and its active form had a molecular mass of 25 kDa. The cDNA of OHSS cloned from an embryonic cDNA library was 1759 bp long, encoding 492 amino acids in a single open reading frame (ORF). The C-terminal part of the predicted protein was composed of a trypsin-like serine protease domain, with homology to counterparts in other animals of 33-38%. The predicted protein (54.7 kDa) secreted as a zymogen may be cleaved post-translationally, separating the C-terminal from the N-terminal region. The OHSS gene was expressed in the embryo at least 2 weeks before hatching. Expression was also detected in the zoea larva 1 day after hatching and in the brain of the female. However, it was not detected in the muscle, hepatopancreas or ovigerous seta of the female. Ultrastructural analysis indicated that the material investing maternal ovigerous hair, i.e. the outermost layer (E1) of the egg case, is attached at the special sites (attachment sites) arranged at intervals of 130-160 nm on the hair. It is suggested that OHSS acts specifically at these sites, lysing the bond with the coat, thus disposing of the embryo attachment system. This enables the female to prepare the next clutch of embryos without ecdysis.

KW - Crab

KW - Embryo attachment system

KW - Investment coat

KW - Ovigerous hair

KW - Ovigerous-hair stripping substance (OHSS)

KW - Serine protease

KW - Sesarma (or Chiromantes) haematocheir

KW - Stripping

UR - http://www.scopus.com/inward/record.url?scp=1242277812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1242277812&partnerID=8YFLogxK

U2 - 10.1242/jeb.00785

DO - 10.1242/jeb.00785

M3 - Article

VL - 207

SP - 621

EP - 632

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 4

ER -