Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the atp operon

Satoshi Wakai, Asami Ohmori, Tadayoshi Kanao, Tsuyoshi Sugio, Kazuo Kamimura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

ATPase was purified 51-fold from a chemoautotrophic, obligately acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans NASF-1. The purified ATPase showed the typical subunit pattern of the F1-ATPase on a polyacrylamide gel containing sodium dodecyl sulfate, with 5 subunits of apparent molecular masses of 55, 50, 33, 20, and 18 kDa. The enzyme hydrolyzed ATP, GTP, and ITP, but neither UTP nor ADP. The Km, value for ATP was 1.8 mM. ATPase activity was optimum at pH 8.5 at 45°C, and was activated by sulfite. Azide strongly inhibited the enzyme activity, whereas the enzyme was relatively resistant to vanadate, nitrate, and N,N′- dicyclohexylcarbodiimide. The genes encoding the subunits for the F 1FO-ATPase from A. ferrooxidans NASF-1 were cloned as three overlapping fragments by PCR cloning and sequenced. The molecular masses of the α, β, γ, δ, and ε subunits of the F 1 portion were deduced from the amino acid sequences to be 55.5, 50.5, 33.1, 19.2, and 15.1 kDa, respectively.

Original languageEnglish
Pages (from-to)1884-1891
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number10
DOIs
Publication statusPublished - Oct 23 2005

Fingerprint

Acidithiobacillus
Acidithiobacillus ferrooxidans
H-transporting ATP synthase
Proton-Translocating ATPases
operon
Operon
Purification
adenosinetriphosphatase
Adenosine Triphosphatases
Molecular mass
Adenosinetriphosphate
Enzymes
Adenosine Triphosphate
molecular weight
Inosine Triphosphate
Dicyclohexylcarbodiimide
azides
Sulfites
Uridine Triphosphate
Gene encoding

Keywords

  • Acidithiobacillus ferrooxidans
  • Acidophile
  • atp operon
  • FF -ATPase

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the atp operon. / Wakai, Satoshi; Ohmori, Asami; Kanao, Tadayoshi; Sugio, Tsuyoshi; Kamimura, Kazuo.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 10, 23.10.2005, p. 1884-1891.

Research output: Contribution to journalArticle

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