Purification and bioactivity of a sulphated polysaccharide conjugate from viscera of abalone haliotis discus hannai Ino

Liming Sun, Beiwei Zhu, Dongmei Li, Lisha Wang, Xiuping Dong, Yoshiyuki Murata, Rong Xing, Ying Dong

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A sulphated polysaccharide conjugate ACP I was purified from the viscera of abalone Haliotis discus hannai Ino, and its immunostimulatory and anti-tumour activities were studied in vivo. Effect of ACP I on immune function was investigated in normal and cyclophosphamide-induced immunosuppressive mice. The anti-tumour effect was investigated by growth inhibition of tumour. Dose-dependent increase in spleen and thymus relative weight, lymphocyte proliferation, phagocytosis of macrophage, natural killer cell activity and antibody production was observed in all the tested mice administered with 10, 20 and 40 mg kg-1 body weight of ACP I. Significant increase of the above function was observed especially in immunosuppressive mice at dose of 40 mg kg-1. A significant inhibition of tumour growth was observed in 10, 20 and 40 mg kg-1 ACP I groups, with inhibitory rates of 40.24, 41.91 and 46.43%, respectively. Results suggested that ACP I might have immunomodulating and anti-tumour potential deserving application in functional food industry.

Original languageEnglish
Pages (from-to)15-26
Number of pages12
JournalFood and Agricultural Immunology
Volume21
Issue number1
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Haliotis discus hannai
Viscera
abalone
animal organs
Polysaccharides
polysaccharides
neoplasms
immunosuppressive agents
Neoplasms
Immunosuppressive Agents
mice
Functional Food
cyclophosphamide
Food Industry
natural killer cells
antibody formation
lymphocyte proliferation
Growth
dosage
phagocytosis

Keywords

  • Abalone viscera
  • Anti-tumour activity
  • Immunomodulation
  • Polysaccharide conjugate
  • Purification

ASJC Scopus subject areas

  • Food Science
  • Immunology
  • Agronomy and Crop Science

Cite this

Purification and bioactivity of a sulphated polysaccharide conjugate from viscera of abalone haliotis discus hannai Ino. / Sun, Liming; Zhu, Beiwei; Li, Dongmei; Wang, Lisha; Dong, Xiuping; Murata, Yoshiyuki; Xing, Rong; Dong, Ying.

In: Food and Agricultural Immunology, Vol. 21, No. 1, 03.2010, p. 15-26.

Research output: Contribution to journalArticle

Sun, Liming ; Zhu, Beiwei ; Li, Dongmei ; Wang, Lisha ; Dong, Xiuping ; Murata, Yoshiyuki ; Xing, Rong ; Dong, Ying. / Purification and bioactivity of a sulphated polysaccharide conjugate from viscera of abalone haliotis discus hannai Ino. In: Food and Agricultural Immunology. 2010 ; Vol. 21, No. 1. pp. 15-26.
@article{44fb0a0021d0492189e7df77ea5bca1c,
title = "Purification and bioactivity of a sulphated polysaccharide conjugate from viscera of abalone haliotis discus hannai Ino",
abstract = "A sulphated polysaccharide conjugate ACP I was purified from the viscera of abalone Haliotis discus hannai Ino, and its immunostimulatory and anti-tumour activities were studied in vivo. Effect of ACP I on immune function was investigated in normal and cyclophosphamide-induced immunosuppressive mice. The anti-tumour effect was investigated by growth inhibition of tumour. Dose-dependent increase in spleen and thymus relative weight, lymphocyte proliferation, phagocytosis of macrophage, natural killer cell activity and antibody production was observed in all the tested mice administered with 10, 20 and 40 mg kg-1 body weight of ACP I. Significant increase of the above function was observed especially in immunosuppressive mice at dose of 40 mg kg-1. A significant inhibition of tumour growth was observed in 10, 20 and 40 mg kg-1 ACP I groups, with inhibitory rates of 40.24, 41.91 and 46.43{\%}, respectively. Results suggested that ACP I might have immunomodulating and anti-tumour potential deserving application in functional food industry.",
keywords = "Abalone viscera, Anti-tumour activity, Immunomodulation, Polysaccharide conjugate, Purification",
author = "Liming Sun and Beiwei Zhu and Dongmei Li and Lisha Wang and Xiuping Dong and Yoshiyuki Murata and Rong Xing and Ying Dong",
year = "2010",
month = "3",
doi = "10.1080/09540100903418859",
language = "English",
volume = "21",
pages = "15--26",
journal = "Food and Agricultural Immunology",
issn = "0954-0105",
publisher = "Taylor and Francis Ltd.",
number = "1",

}

TY - JOUR

T1 - Purification and bioactivity of a sulphated polysaccharide conjugate from viscera of abalone haliotis discus hannai Ino

AU - Sun, Liming

AU - Zhu, Beiwei

AU - Li, Dongmei

AU - Wang, Lisha

AU - Dong, Xiuping

AU - Murata, Yoshiyuki

AU - Xing, Rong

AU - Dong, Ying

PY - 2010/3

Y1 - 2010/3

N2 - A sulphated polysaccharide conjugate ACP I was purified from the viscera of abalone Haliotis discus hannai Ino, and its immunostimulatory and anti-tumour activities were studied in vivo. Effect of ACP I on immune function was investigated in normal and cyclophosphamide-induced immunosuppressive mice. The anti-tumour effect was investigated by growth inhibition of tumour. Dose-dependent increase in spleen and thymus relative weight, lymphocyte proliferation, phagocytosis of macrophage, natural killer cell activity and antibody production was observed in all the tested mice administered with 10, 20 and 40 mg kg-1 body weight of ACP I. Significant increase of the above function was observed especially in immunosuppressive mice at dose of 40 mg kg-1. A significant inhibition of tumour growth was observed in 10, 20 and 40 mg kg-1 ACP I groups, with inhibitory rates of 40.24, 41.91 and 46.43%, respectively. Results suggested that ACP I might have immunomodulating and anti-tumour potential deserving application in functional food industry.

AB - A sulphated polysaccharide conjugate ACP I was purified from the viscera of abalone Haliotis discus hannai Ino, and its immunostimulatory and anti-tumour activities were studied in vivo. Effect of ACP I on immune function was investigated in normal and cyclophosphamide-induced immunosuppressive mice. The anti-tumour effect was investigated by growth inhibition of tumour. Dose-dependent increase in spleen and thymus relative weight, lymphocyte proliferation, phagocytosis of macrophage, natural killer cell activity and antibody production was observed in all the tested mice administered with 10, 20 and 40 mg kg-1 body weight of ACP I. Significant increase of the above function was observed especially in immunosuppressive mice at dose of 40 mg kg-1. A significant inhibition of tumour growth was observed in 10, 20 and 40 mg kg-1 ACP I groups, with inhibitory rates of 40.24, 41.91 and 46.43%, respectively. Results suggested that ACP I might have immunomodulating and anti-tumour potential deserving application in functional food industry.

KW - Abalone viscera

KW - Anti-tumour activity

KW - Immunomodulation

KW - Polysaccharide conjugate

KW - Purification

UR - http://www.scopus.com/inward/record.url?scp=76549092477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76549092477&partnerID=8YFLogxK

U2 - 10.1080/09540100903418859

DO - 10.1080/09540100903418859

M3 - Article

AN - SCOPUS:76549092477

VL - 21

SP - 15

EP - 26

JO - Food and Agricultural Immunology

JF - Food and Agricultural Immunology

SN - 0954-0105

IS - 1

ER -