TY - JOUR
T1 - Purification and bioactivity of a sulphated polysaccharide conjugate from viscera of abalone haliotis discus hannai Ino
AU - Sun, Liming
AU - Zhu, Beiwei
AU - Li, Dongmei
AU - Wang, Lisha
AU - Dong, Xiuping
AU - Murata, Yoshiyuki
AU - Xing, Rong
AU - Dong, Ying
N1 - Funding Information:
This work was financially supported by National High Technology Research and Development Programme of China (No. 2007AA091804), National Science & Technology Pillar Programme in the Eleventh Five-year Plan Period of China (No. 2008BAD94B07; No. 2008BAD94B06), Innovative Research Team Project (No. 2008T007) and Key Subject of Food Science and Engineering of Zhejiang Province, China (No. ZSZKF200714). We thank Dayong Zhou, Lili Zhu and Meiling Xu for technical help.
PY - 2010/3
Y1 - 2010/3
N2 - A sulphated polysaccharide conjugate ACP I was purified from the viscera of abalone Haliotis discus hannai Ino, and its immunostimulatory and anti-tumour activities were studied in vivo. Effect of ACP I on immune function was investigated in normal and cyclophosphamide-induced immunosuppressive mice. The anti-tumour effect was investigated by growth inhibition of tumour. Dose-dependent increase in spleen and thymus relative weight, lymphocyte proliferation, phagocytosis of macrophage, natural killer cell activity and antibody production was observed in all the tested mice administered with 10, 20 and 40 mg kg-1 body weight of ACP I. Significant increase of the above function was observed especially in immunosuppressive mice at dose of 40 mg kg-1. A significant inhibition of tumour growth was observed in 10, 20 and 40 mg kg-1 ACP I groups, with inhibitory rates of 40.24, 41.91 and 46.43%, respectively. Results suggested that ACP I might have immunomodulating and anti-tumour potential deserving application in functional food industry.
AB - A sulphated polysaccharide conjugate ACP I was purified from the viscera of abalone Haliotis discus hannai Ino, and its immunostimulatory and anti-tumour activities were studied in vivo. Effect of ACP I on immune function was investigated in normal and cyclophosphamide-induced immunosuppressive mice. The anti-tumour effect was investigated by growth inhibition of tumour. Dose-dependent increase in spleen and thymus relative weight, lymphocyte proliferation, phagocytosis of macrophage, natural killer cell activity and antibody production was observed in all the tested mice administered with 10, 20 and 40 mg kg-1 body weight of ACP I. Significant increase of the above function was observed especially in immunosuppressive mice at dose of 40 mg kg-1. A significant inhibition of tumour growth was observed in 10, 20 and 40 mg kg-1 ACP I groups, with inhibitory rates of 40.24, 41.91 and 46.43%, respectively. Results suggested that ACP I might have immunomodulating and anti-tumour potential deserving application in functional food industry.
KW - Abalone viscera
KW - Anti-tumour activity
KW - Immunomodulation
KW - Polysaccharide conjugate
KW - Purification
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U2 - 10.1080/09540100903418859
DO - 10.1080/09540100903418859
M3 - Article
AN - SCOPUS:76549092477
VL - 21
SP - 15
EP - 26
JO - Food and Agricultural Immunology
JF - Food and Agricultural Immunology
SN - 0954-0105
IS - 1
ER -