An experimental investigation was conducted to reveal jet penetration and mixing performance of pulsed injection in a Mach 2.5 crossflow. Helium and nitrogen gas were injected perpendicularly through flush-mounted circular sonic orifice. Probing techniques including species composition sampling and high speed framing schlieren were employed to determine the penetration and mixing performance at several downstream locations. Our investigation consisted essentially of two parts. The first part was an investigation of the continuous jet. The performance of the continuous jet was mainly controlled by effective velocity ratio (r) as the square root of the momentum-flux ratio and the orifice diameter (d). The centerline trajectories of the jets and the maximum concentration decay were collapsed by the rd scale and the ratio of oncoming boundary-layer thickness to the injector diameter. The second part was an comparison of the performance between the pulse and continuous jets. The penetration of the pulse jet was adjustable by changing the pulse duty cycle at a condition of fixed injectant mass flow rate. Even at a condition of fixed injection pressure, the pulsed injection showed better mixing performance and the higher penetration, due to the fluctuation of the large-scale eddies in the jet associated with the fluctuation of the bow shock in front of the jet.