Pulsed-field gel electrophoresis typing of multidrug-resistant Vibrio parahaemolyticus isolated from various sources of seafood

Fang Liu, Wenying Guan, M. J. Alam, Zhixin Shen, Shuhong Zhang, Lin Li, Sumio Shinoda, Lei Shi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Vibrio parahaemolyticus (V. parahaemolyticus), one of the most important foodborne pathogens in many maritime Asian countries, is frequently associated with the consumption of seafood. Thirty eight strains of V. parahaemolyticus were isolated from seafood in Hebei province of China. Resistance to 13 antibiotics was determined using broth microdilution methods. These strains were typed by pulsed-field gel electrophoresis (PFGE) technique with short pre-processing following SfiI digestion and a typing scheme was generated. The 38 strains were grouped into 5 types with 71% pattern similarity. All the type E were isolated from Shijiazhuang, Baoding and Langfang and simultaneously resistant to ampicillin, sulfisoxazole, streptonigrin and vancomycin, suggesting a clonal relationship between these strains. The data of antimicrobial susceptibility test and PFGE profiles in this study showed a good correlation among antimicrobial susceptibility test, PFGE profiles and geographic distribution.

Original languageEnglish
Pages (from-to)783-789
Number of pages7
JournalJournal of Health Science
Volume55
Issue number5
DOIs
Publication statusPublished - Oct 2009
Externally publishedYes

Fingerprint

Vibrio parahaemolyticus
Seafood
Pulsed Field Gel Electrophoresis
Electrophoresis
Gels
Streptonigrin
Sulfisoxazole
Pathogens
Vancomycin
Ampicillin
Digestion
China
Anti-Bacterial Agents
Processing

Keywords

  • Antimicrobial resistance
  • Pulsed field gel electrophoresis
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Pulsed-field gel electrophoresis typing of multidrug-resistant Vibrio parahaemolyticus isolated from various sources of seafood. / Liu, Fang; Guan, Wenying; Alam, M. J.; Shen, Zhixin; Zhang, Shuhong; Li, Lin; Shinoda, Sumio; Shi, Lei.

In: Journal of Health Science, Vol. 55, No. 5, 10.2009, p. 783-789.

Research output: Contribution to journalArticle

Liu, Fang ; Guan, Wenying ; Alam, M. J. ; Shen, Zhixin ; Zhang, Shuhong ; Li, Lin ; Shinoda, Sumio ; Shi, Lei. / Pulsed-field gel electrophoresis typing of multidrug-resistant Vibrio parahaemolyticus isolated from various sources of seafood. In: Journal of Health Science. 2009 ; Vol. 55, No. 5. pp. 783-789.
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