PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

Dongru Qiu, F. Heath Damron, Takehiko Mima, Herbert P. Schweizer, Hongwei D. Yu

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

Original languageEnglish
Pages (from-to)7422-7426
Number of pages5
JournalApplied and environmental microbiology
Volume74
Issue number23
DOIs
Publication statusPublished - Dec 1 2008

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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